Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat
Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat
复制标题
中间堇草抗白粉病基因Pm43基因渗入小麦的遗传与定位
DOI：
10.1007/s00122-009-0971-z
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发表时间：
2009-04-01
影响因子：
5.4
通讯作者：
Liu, Jianxia
中科院分区：
文献类型：
--
作者：
He, Runli;Chang, Zhijian;Liu, Jianxia
将中间偃麦草抗白粉病基因导入普通小麦中。对CH 5025的F-1、F-2、F-3和BC 1群体的遗传分析表明，抗性受一个显性等位基因控制。通过对分离的F-2群体的连锁分析，定位了与白粉病抗性相关的基因。该抗病基因与5个共显性SSR标记（Xcfd 233、Xwmc 41、Xbarc 11、Xgwm 539和Xwmc 175）连锁，其最可能的连锁顺序分别为Xcfd 233-Xwmc 41-Pm 43-Xbarc 11-Xgwm 539-Xwmc 175，连锁距离分别为2.6、2.3、4.2、3.5和7.0cM。利用中国春缺体-四体和双端体材料，将多态性标记和抗病基因定位在2DL染色体上。由于之前没有将抗白粉病基因分配到染色体2DL上，因此将该新的抗白粉病基因命名为Pm 43。pm 43与已鉴定的紧密连锁标记一起可用于分子标记辅助选择抗白粉病基因。
Powdery mildew resistance from Thinopyrum intermedium was introgressed into common wheat (Triticum aestivum L.). Genetic analysis of the F-1, F-2, F-3 and BC1 populations from powdery mildew resistant line CH5025 revealed that resistance was controlled by a single dominant allele. The gene responsible for powdery mildew resistance was mapped by the linkage analysis of a segregating F-2 population. The resistance gene was linked to five co-dominant genomic SSR markers (Xcfd233, Xwmc41, Xbarc11, Xgwm539 and Xwmc175) and their most likely order was Xcfd233-Xwmc41-Pm43-Xbarc11-Xgwm539-Xwmc175 at 2.6, 2.3, 4.2, 3.5 and 7.0 cM, respectively. Using the Chinese Spring nullisomic-tetrasomic and ditelosomic lines, the polymorphic markers and the resistance gene were assigned to chromosome 2DL. As no powdery mildew resistance gene was previously assigned to chromosome 2DL, this new resistance gene was designated Pm43. Pm43, together with the identified closely linked markers, could be useful in marker-assisted selection for pyramiding powdery mildew resistance genes.