Meox1Cre:: A mouse line expressing cre recombinase in somitic mesoderm
Meox1Cre:: A mouse line expressing cre recombinase in somitic mesoderm
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DOI：
10.1002/gene.20163
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发表时间：
2005-11-01
期刊：
影响因子：
1.5
通讯作者：
Partanen, J
中科院分区：
文献类型：
--
作者：
Jukkola, T;Trokovic, R;Partanen, J
我们开发了一种新的策略的基础上，在体外DNA转座的噬菌体Mu构建载体的“敲入”的基因编码Cre重组酶到内源性位点的胚胎干细胞。该策略被用于将Cre引入小鼠Meox 1基因座，预期其将驱动Cre在体前和体中胚层中的表达。在Meox 1（Cre）和R26 R或Z/AP报告等位基因杂合的胚胎中，在成熟的体节及其衍生物中检测到报告等位基因的特异性和有效重组，包括发育中的椎骨，骨骼肌，背部真皮，以及侵入脊髓和发育中的肢体的血管内皮。在体细胞中胚层，Cre活性没有观察到在颅旁轴中胚层。因此，Meox 1（Cre）等位基因允许详细的Meox 1表达组织，包括体细胞中胚层的衍生物的命运映射。我们用它来证明动态变化的组成间充质周围的发展内耳。Meox 1（Cre）也可用于体节中胚层及其衍生物中的组织特异性诱变。
We developed a novel strategy based on in vitro DNA transposition of phage Mu to construct vectors for "knock-in" of the gene encoding Cre recombinase into endogenous loci in embryonic stem cells. This strategy was used to introduce Cre into the mouse Meox1 locus, which was expected to drive Cre expression in the presomitic and somitic mesoderm. In embryos heterozygous for both Meox1(Cre) and R26R or Z/AP reporter alleles, specific and efficient recombination of the reporter alleles was detected in the maturing somites and their derivatives, including developing vertebrae, skeletal muscle, back dermis, as well as endothelium of the blood vessels invading the spinal cord and developing limbs. In contrast to the somitic mesoderm, Cre activity was not observed in the cranial paraxial mesoderm. Thus, the Meox1(Cre) allele allows detailed fate-mapping of Meox1-expressing tissues, including derivatives of the somitic mesoderm. We used it to demonstrate dynamic changes in the composition of the mesenchyme surrounding the developing inner ear. Meox1(Cre) may also be used for tissue-specific mutagenesis in the somitic mesoderm and its derivatives.