Associations of high-risk HPV types and viral load with cervical cancer in China
Associations of high-risk HPV types and viral load with cervical cancer in China
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DOI：
10.1016/j.jcv.2005.07.011
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发表时间：
2006-03-01
影响因子：
8.8
通讯作者：
He, Y
中科院分区：
文献类型：
--
作者：
Wu, YP;Chen, YL;He, Y
背景：宫颈癌是全世界女性第二常见的癌症。人乳头瘤病毒某些基因型的感染是与宫颈癌相关的最重要的危险因素。 目的：本研究旨在确定我国HPV的流行情况和基因型，并评估高危型HPV病毒载量与宫颈癌及其癌前病变的相关性。 研究设计：采用横断面研究，采集541例宫颈癌患者的宫颈样本。 宫颈癌、262 名 CIN、139 名宫颈炎和 68 名年龄匹配的健康对照。 Hybrid Capture 2 用于检测 HPV DNA。使用类型特异性 PCR 和基于序列分型的通用引物 PCR (GP PCR-SBT) 对 HPV DNA 阳性宫颈癌标本进行 HPV 类型检测。结果：总体高危 HPV 患病率为 CIN1 中 68.8%、CIN2 中 80.3%、CIN3 中 90.2%、原位宫颈癌 90.9%、浸润性宫颈癌 89.9% 和 25% 来自中国的健康控制。宫颈癌患者中最常见的HPV DNA类型是HPV 16（79.6％），其次是HPV58（5.92％），HPV33（3.29％），HPV18（1.97％），HPV6（1.97％），HPV31（1.31％）HPV39（1.31％），HPV68（1.31％）和其他HPV类型 （3.3%）。结果发现，病毒载量高时，CIN 阶段增加的风险显着增加。对照中发现的低病毒载量频率为 CIN1 的 13.2% 和 22.9%，OR 为 4.2 (1.5-12.0)。低病毒载量与 CIN2/3、CIS 和 CC 之间的关联 (OR) 分别为 6.7 (2.9-15.6)、9.4 (2.7-32.3) 和 8.3 (3.7-18.4)。 5.9% 的对照、27.1% 的 CIN1、42.1% 的 CIN2/3 和 48.5% 的 CIS 发现高病毒载量，表明比值比随着疾病严重程度的增加而增加（CIS 的 OR = 68.0，95% CI = 17.8-259.7）。结论：HPV16 是中国中部地区最常见的基因型。宫颈癌前兆的发生与高危 HPV 病毒载量升高有关。 (c) 2005 Elsevier B.V. 保留所有权利。
Background: Cervical cancer is the second most common cancer in women worldwide. Infection with some genotypes of human papillomavirus is the most important risk factor associated with cervical cancer.Objective: The aim of this study was to determine the prevalence and genotypes of HPV in China, and to evaluate the correlation between viral load of high risk HPV and cervical cancer and its precursors.Study design: A cross-sectional study was carried out, wherein cervical samples were collected from 541 patients with cervical cancer, 262 with CIN, 139 with cervicitis and 68 age-matched healthy controls. Hybrid Capture 2 was employed to detect HPV DNA. Specimens from HPV DNA positive cervical cancer were tested for HPV types by using type specific PCR and general primer PCR with sequence-based typing (GP PCR-SBT).Results: Overall high risk HPV prevalence was 68.8% in CIN1 80.3% in CIN2, 90.2% in CIN3, 90.9% in cervical cancer in situ, 89.9% in invasive cervical cancer and 25% in healthy controls from China. The most common HPV DNA type found in patients with cervical cancer was HPV 16 (79.6%), followed by HPV58 (5.92%), HPV33 (3.29%), HPV18 (1.97%), HPV6 (1.97%), HPV31 (1.31%) HPV39 (1.31%), HPV68(1.31%) and other HPV types (3.3%). It was found that there was a significantly increased risk of increasing CIN stage with high viral load. Frequency of low viral load found in the controls was 13.2% and 22.9% of CIN1, obtaining an OR of 4.2 (1.5-12.0). Associations (OR) among low viral load and CIN2/3, CIS, and CC were 6.7 (2.9-15.6), 9.4 (2.7-32.3) and 8.3 (3.7-18.4), respectively. While high viral loads were found in 5.9% of controls, 27.1% of CIN1, 42.1% of CIN2/3 and 48.5% of CIS, demonstrating increasing odds ratios with severity of disease (OR for CIS = 68.0, 95% CI = 17.8-259.7).Conclusions: HPV16 was the most common genotype in central China. The developing cervical cancer precursors were associated with elevated high-risk HPV viral load. (c) 2005 Elsevier B.V. All rights reserved.