Circular RNA expression profiling in the nucleus accumbens: Effects of electroacupuncture treatment on morphine-induced conditioned place preference
Circular RNA expression profiling in the nucleus accumbens: Effects of electroacupuncture treatment on morphine-induced conditioned place preference
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伏隔核中的环状RNA表达谱：电针治疗对吗啡诱导的条件性位置偏好的影响
DOI：
10.1111/adb.12794
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发表时间：
2019
影响因子：
3.4
通讯作者：
Zhigang Lu
中科院分区：
文献类型：
--
作者：
Han Zhang;Qian Wang;Qisheng Wang;Anlong Liu;Fenfen Qin;Qinmei Sun;Qian Li;Yun Gu;Zongxiang Tang;Shengfeng Lu;Zhigang Lu
电针是在中国传统针灸的基础上发展起来的。电针可抑制阿片成瘾者的渴求和啮齿动物的阿片寻找反应。然而，电针对吗啡奖赏特性和渴求反应的分子机制尚不清楚。在这里，我们在小鼠身上应用了条件性位置偏爱范式来测量吗啡诱导的奖赏效应以及电针治疗。环状RNA(CircRNAs)可以作为微小RNA(MiRNA)海绵有效地调节基因表达水平。在电针治疗和假治疗的小鼠中，进行了伏隔核(NAC)内的CircRNA分析。在RNAseq之后，使用基因本体论(GO)和京都百科全书基因和基因组(KEGG)工具进行数据分析。我们发现了112个差异表达的CircRNA，其中51个上调，61个下调。我们的生物信息学分析表明，这些差异表达的CircRNAs映射到主要参与肾素分泌和cGMP-PKG信号转导的途径。我们进一步构建了CircRNA-miRNA网络，用于预测差异表达的CircRNAs的潜在作用以及CircRNAs与miRNAs的相互作用。我们在电针治疗吗啡诱导的CPP后对NAC进行的二级测序和生物信息学分析为研究吗啡强化和可能的渴求的分子机制提供了可能的新靶点。
Electroacupuncture (EA) has been developed on the basis of traditional Chinese acupuncture. EA can suppress craving in opioid addicts and opioid‐seeking responses in rodents. However, the molecular mechanism of EA on the rewarding properties of morphine and craving responses is not known. Here, we have applied a conditioned place preference paradigm in mice to measure morphine‐induced rewarding effects along with EA treatment. Circular RNAs (circRNAs) can function as micro RNA (miRNA) sponges to effectively regulate gene expression levels. CircRNA profiling within the nucleus accumbens (NAc) was performed in EA‐treated and sham‐treated mice. Following RNAseq, data were analyzed by gene ontology (GO) and Kyoto Encyclopedia Genes and Genomes (KEGG) tools. We identified 112 significantly differentially expressed circRNAs, including 51 that were up‐regulated and 61 that were down‐regulated. Our bioinformatics analyses show that these differentially expressed circRNAs map into pathways that are mainly involved with renin secretion and the cGMP‐PKG signaling. We further constructed a circRNA‐miRNA network that predicts the potential roles of the differentially expressed circRNAs and the interaction of circRNAs with miRNAs. Our secondary sequencing and bioinformatics analysis in the NAc after EA treatment on morphine‐induced CPP provides putative novel targets on molecular mechanisms involved in morphine reinforcement and possibly craving.