STRUCTURES OF ACIDIC O-LINKED POLYLACTOSAMINOGLYCANS ON HUMAN SKIM MILK MUCINS
STRUCTURES OF ACIDIC O-LINKED POLYLACTOSAMINOGLYCANS ON HUMAN SKIM MILK MUCINS
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DOI：
10.1007/bf01189075
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
UHLENBRUCK, G
中科院分区：
文献类型：
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作者：
HANISCH, FG;PETERKATALINIC, J;UHLENBRUCK, G
从人脱脂乳粘蛋白或粘蛋白衍生的高分子量糖肽中分离O-连接聚糖，并通过阴离子交换色谱法将其分离为中性和酸性糖醇。通过高效液相色谱法纯化酸性馏分中所含的主要寡糖，并通过快速原子轰击质谱法，甲基化分析和500 MHz 1H-核磁共振光谱法的组合进行结构表征。酸性部分中这些主要物质表现出的结构方面类似于先前对来自人脱脂乳粘蛋白的中性寡糖建立的那些：1）糖醇的大小从三糖醇到十糖醇变化2）核心结构是普遍存在的类型23）主链序列是聚-N-乙酰乳糖胺类型，具有线性延伸的GlcNAcβ（1-3）Gal（主要）或GlcNAcβ（1-6）Gal单元（次要）的特别优势。单唾液酸化（粘蛋白或糖肽衍生）和二唾液酸化聚糖（糖肽衍生）上的N-乙酰神经氨酸主要连接到半乳糖的C-3位。
O-Linked glycans were isolated from human skim milk mucins or mucin-derived high-molecular weight glycopeptides and fractionated by anion exchange chromatography into neutral and acidic alditols. Major oligosaccharides contained in the acidic fraction were purified by high performance liquid chromatography and structurally characterized by a combination of fast atom bombardment mass spectrometry, methylation analysis and 500 MHz1H-nuclear magnetic resonance spectroscopy. The structural aspects exhibited by these major species in the acidic fraction resemble those established previously for the neutral oligosaccharides from human skim milk mucins:1)the size of the alditols varies from tri- to decasaccharides2)the core structure is of the ubiquitous type 23)the backbone sequences are of the poly-N-acetyllactosamine type with a particular preponderance of linearly extended GlcNAcβ(1–3)Gal (major) or GlcNAcβ(1–6)Gal units (minor).N-Acetylneuraminic acid on monosialylated (mucin- or glycopeptide-derived) and disialylated glycans (glycopeptide derived) is linked predominantly to position C-3 of galactose.