Sensory deprivation differentially impacts the dendritic development of pyramidal versus non-pyramidal neurons in layer 6 of mouse barrel cortex
Sensory deprivation differentially impacts the dendritic development of pyramidal versus non-pyramidal neurons in layer 6 of mouse barrel cortex
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DOI：
10.1007/s00429-011-0342-9
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发表时间：
2012-04-01
影响因子：
3.1
通讯作者：
Brumberg, Joshua C.
中科院分区：
文献类型：
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作者：
Chen, Chia-Chien;Tam, Danny;Brumberg, Joshua C.
出生后早期的感觉经验对影响行为的皮层回路的结构和功能有深远的影响。使用小鼠胡须到桶系统，我们长期剥夺动物的正常感觉经验，从出生后的第一个月开始，每隔一天双侧修剪它们的胡须。然后处理脑组织进行高尔基体染色，并在三维中重建桶皮质第6层中的神经元。树突和躯体参数之间的感觉剥夺和正常的感觉经验组进行了比较。结果表明，在慢性剥夺组的第6层非锥体神经元表现出扩张的树突状乔木。锥体细胞对感觉剥夺的反应是躯体大小和基底树突分支增加，但总体上顶端树突参数降低。总之，感觉剥夺影响了第6层锥体和非锥体神经元的神经元结构，这可能为观察到的胡须修剪引起的生理和行为变化提供了基础。
Early postnatal sensory experience can have profound impacts on the structure and function of cortical circuits affecting behavior. Using the mouse whisker-to-barrel system we chronically deprived animals of normal sensory experience by bilaterally trimming their whiskers every other day from birth for the first postnatal month. Brain tissue was then processed for Golgi staining and neurons in layer 6 of barrel cortex were reconstructed in three dimensions. Dendritic and somatic parameters were compared between sensory-deprived and normal sensory experience groups. Results demonstrated that layer 6 non-pyramidal neurons in the chronically deprived group showed an expansion of their dendritic arbors. The pyramidal cells responded to sensory deprivation with increased somatic size and basilar dendritic arborization but overall decreased apical dendritic parameters. In sum, sensory deprivation impacted on the neuronal architecture of pyramidal and non-pyramidal neurons in layer 6, which may provide a substrate for observed physiological and behavioral changes resulting from whisker trimming.