The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling
The two biosynthetic routes leading to phosphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling
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DOI：
10.1021/bi026801r
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发表时间：
2003-03-18
期刊：
影响因子：
2.9
通讯作者：
de Kroon, AIPM
中科院分区：
文献类型：
--
作者：
Boumann, HA;Damen, MJA;de Kroon, AIPM
磷脂酰胆碱（PC）是真核生物膜上的一类主要脂类，可通过磷脂酰乙醇胺（PE）的三甲基化或cdp -胆碱途径合成。为了研究这两种生物合成途径是否对PC物种的稳定状态有不同的贡献，即具有特定酰基链组成的PC分子，我们用两种途径的氘化前体（甲基- d -3）-蛋氨酸和(D-13)-胆碱分别标记酿酒酵母，对新合成的PC物种池进行了监测。电喷雾电离串联质谱分析（ESI-MS/MS）结果表明，两种合成途径产生不同种类的PC，其中cdp -胆碱途径对PC的分子多样性贡献最大。此外，酵母被证明能够通过甘油主链sn-1位置的酰基链交换来重塑PC。发现重塑需要产生稳态的PC物种分布。这是首次证明酵母中两种生物合成途径之间功能差异的研究。
Phosphatidylcholine (PC), a major lipid class in the membranes of eukaryotes, is synthesized either via the triple methylation of phosphatidylethanolamine (PE) or via the CDP-choline route. To investigate whether the two biosynthetic routes contribute differently to the steady-state profile of PC species, i.e., PC molecules with specific acyl chain compositions, the pools of newly synthesized PC species were monitored by labeling Saccharomyces cerevisiae with deuterated precursors of the two routes, (methyl-D-3)-methionine and (D-13)-choline, respectively. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) revealed that the two PC biosynthetic pathways yield different sets of PC species, with the CDP-choline route contributing most to the molecular diversity. Moreover, yeast was shown to be capable of remodeling PC by acyl chain exchange at the sn-1 position of the glycerol backbone. Remodeling was found to be required to generate the steady-state species distribution of PC. This is the first study demonstrating a functional difference between the two biosynthetic routes in yeast.