ANTIADHESIVE ACTIVITY OF HUMAN CASEIN AGAINST STREPTOCOCCUS-PNEUMONIAE AND HAEMOPHILUS-INFLUENZAE
ANTIADHESIVE ACTIVITY OF HUMAN CASEIN AGAINST STREPTOCOCCUS-PNEUMONIAE AND HAEMOPHILUS-INFLUENZAE
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DOI：
10.1016/0882-4010(90)90090-d
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发表时间：
1990-05-01
影响因子：
3.8
通讯作者：
SVANBORG, C
中科院分区：
文献类型：
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作者：
ANIANSSON, G;ANDERSSON, B;SVANBORG, C
发现人乳的酪蛋白组分抑制肺炎链球菌和流感嗜血杆菌人呼吸道上皮细胞的附着。对S.在酪蛋白的热和胰蛋白酶处理后，肺炎链球菌保留，并且在从酪蛋白释放的寡糖中发现。.卡帕-酪蛋白是糖基化程度最高的酪蛋白成分，在与全酪蛋白组分相似的浓度下可抑制肺炎球菌附着。该结果与已知的S.肺炎，因为人乳和牛初乳，其中含有GlcNAc，抑制附着，但成熟的牛乳缺乏GlcNAc没有。对H.流感病毒与S. pneumoniae，因为附着被人酪蛋白和牛初乳抑制，但不被成熟牛乳或牛酪蛋白组分抑制。卡帕人乳中的酪蛋白成分对H.流感病毒的附着比整个酪蛋白部分和游离寡糖是无活性的。人酪蛋白的这种抗微生物作用代表了母乳预防呼吸道感染的新机制。
The casein fraction of human milk was found to inhibit the attachment of Streptococcus pneumoniae and Haemophilus influenzae human respiratory tract epithelial cells. The inhibitory activity for S. pneumoniae remained after heat and trypsin treatment of the casein and was found in oligosaccharides released from casein. .kappa.-Casein, which is the most highly glycosylated casein component, inhibited pneumococcal attachment at concentrations similar to the whole casein fraction. The results are consistent with the known recognition of GlcNac.beta.1-3Gal by S. pneumoniae, since human milk and bovine colostrum, which contain GlcNAc, inhibited attachment, but mature bovine milk lacking GlcNAc did not. The effect on H. influenza was similar to that on S. pneumoniae in that the attachment was inhibited by human casein and bovine colostrum, but not by either mature bovine milk or by the bovine casein fraction. The .kappa.-casein component of human milk was a less efficient inhibitor of H. influenzae attachment than the whole casein fraction and the free oligosaccharides were inactive. This anti-microbial effect of human casein represents a new mechanism for the protection by breast-milk against respiratory tract infection.