MODEL FOR AMPEROMETRIC ENZYME ELECTRODE OBTAINED THROUGH DIGITAL-SIMULATION AND APPLIED TO IMMOBILIZED GLUCOSE OXIDASE SYSTEM
MODEL FOR AMPEROMETRIC ENZYME ELECTRODE OBTAINED THROUGH DIGITAL-SIMULATION AND APPLIED TO IMMOBILIZED GLUCOSE OXIDASE SYSTEM
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DOI：
10.1021/ac60352a006
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发表时间：
1975-01-01
影响因子：
7.4
通讯作者：
MALOY, JT
中科院分区：
文献类型：
--
作者：
MELL, LD;MALOY, JT
采用数字仿真方法对电流型酶电极的稳态电流响应进行了建模。两种类型的校准曲线的预测，这取决于电流是否是由酶催化或扩散控制。线性校准曲线预测在低底物浓度;然而，线性延伸到浓度大于Km的酶的扩散控制的情况下。这种行为已被观察到使用葡萄糖氧化酶电极会议的扩散控制标准-RIA建立的模拟。稳态数据的方法允许膜介质中葡萄糖的D估计为0.24 × 10 - 5cm 2/sec。观察到的低表观电流密度归因于膜中酶活性的抑制和膜基质的有效电极面积的减少;发现每一个都减少到约1000。在固定化过程中，其理论值的1%。固定化酶电极，是特定的各种底物在这个时候使用。在大多数情况下，通过电位法监测酶反应产生的变化（1-4）。固定化研究中常用的底物-酶组合，
Digital simulation has been used to model the steady-state current response of the amperometric enzyme electrode. Two types of calibration curves are predicted, depending upon whether the current is controlled by enzyme catalysis or by diffusion. Linear calibration curves are predicted at low substrate concentration; however, linearity extends to concentrations greater than Km for the enzyme in the case of diffusion-control. This behavior has been observed using a glucose oxidase electrode meeting diffusion-control crite-ria established by the simulation. Approach to steady-state data permits D for glucose in the membrane medium to be estimated at 0.24 X 10~ 5 cm2/sec. Low apparent current densities observed are attributed to the inhibition of enzyme activity in the membrane and the reduction of effective electrode area by the membrane matrix; each is found to be reduced to ca. 1% of its theoretical valueby the immobilization process.Immobilized enzyme electrodes that are specific for a wide variety of substrates are used at this time. In most cases, changes occurring as a result of the enzyme reaction are monitored potentiometrically (1-4). A frequently used substrate-enzyme combination for the study of immobil-