Intracellular DNA replication and long-term survival of pathogenic mycoplasmas
Intracellular DNA replication and long-term survival of pathogenic mycoplasmas
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DOI：
10.1006/mpat.2000.0395
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发表时间：
2000-11-01
影响因子：
3.8
通讯作者：
Baseman, JB
中科院分区：
文献类型：
--
作者：
Dallo, SF;Baseman, JB
我们研究了致病性支原体（反式支原体、肺炎支原体和生殖支原体）在培养的人类细胞中的细胞内存活和生长。利用真核细胞核DNA合成抑制剂aphidicolin检测支原体（Mycoplasma，My）和线粒体（Mitochondrial，Mt）DNA的选择性合成。胞内M. pneumoniae和M.采用感染细胞亚分级和支原体集落形成单位（cfu）测定方法，在6个月内检测人细胞的体外反式感染性。支原体生殖器，我们未能重新从感染的细胞生长，种特异性PCR引物被用来牵连长期支原体生存能力。数据表明，致病性支原体在人类细胞中长时间驻留并在细胞内复制，这与支原体规避抗生素治疗和免疫监视并建立慢性感染的能力一致。(C)北京大学出版社.
We examined intracellular survival and growth of pathogenic mycoplasmas (Mycoplasma penetrans, Mycoplasma pneumoniae and Mycoplasma genitalium) in cultured human cells. By using the eukaryotic nuclear DNA synthesis inhibitor, aphidicolin, we detected the selective synthesis of mycoplasma (My) and mitochondria (Mt) DNA, which could be further differentiated by restriction enzyme analyses. Also, intracellular M. pneumoniae and M. penetrans infectivity of human cells was detected over 6 months using subfractionation of infected cells and determination of mycoplasma colony forming units (cfu). For M. genitalium, which we failed to re-grow from infected cells, species-specific PCR primers were used to implicate long-term mycoplasma survivability. Data indicated that pathogenic mycoplasmas reside and replicate intracellularly over extended periods in human cells, consistent with the ability of mycoplasmas to circumvent antibiotic therapy and immune surveillance and establish chronic infections. (C) 2000 Academic Press.