ONTOGENY AND FUNCTION OF 2 NONLYMPHOID CELL-POPULATIONS IN THE CHICKEN-EMBRYO
ONTOGENY AND FUNCTION OF 2 NONLYMPHOID CELL-POPULATIONS IN THE CHICKEN-EMBRYO
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DOI：
10.1016/s0171-2985(11)80211-1
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发表时间：
1991-08-01
期刊：
影响因子：
2.8
通讯作者：
JEURISSEN, SHM
中科院分区：
文献类型：
--
作者：
JANSE, EM;JEURISSEN, SHM
该研究的目的是确定在个体发育过程中鸡胚胎中发育的非淋巴细胞类型，以及这些细胞是否具有功能。为了检测这些细胞，我们使用了对两组非淋巴细胞特异性的单克隆抗体：CVI-ChNL-68.1，对单核吞噬细胞特异性，CVI-ChNL-68.2，对脾、肝和囊中的网状细胞亚群特异性。分别对白细胞、B淋巴细胞和IgM具有特异性的单克隆抗体HIS-C7、HIS-C1和HIS-C-12用于关联非淋巴细胞和淋巴细胞的个体发生。在肝脏、脾脏、卵黄囊、囊、肠和胸腺中检测到单核吞噬细胞和网状细胞的时间与白细胞的时间大致相同，但早于B淋巴细胞。此外，酸性磷酸酶被用作吞噬活性的标志物。肝和脾中的网状细胞从检测的第一点起就具有功能，而肝和脾中的单核吞噬细胞在其发育后几天开始吸收抗原。
The purpose of the study was to determine what type of non-lymphoid cells develop in chicken embryos during ontogeny, and whether these cells are functional. To detect these cells, we used monoclonal antibodies specific to two groups of non-lymphoid cells: CVI-ChNL-68.1, specific for mononuclear phagocytes, and CVI-ChNL-68.2, specific for a subpopulation of reticulum cells in spleen, liver and bursa. Monoclonal antibodies HIS-C7, HIS-C1, and HIS-C-12, which are specific to leukocytes, B lymphocytes, and IgM respectively, were used to correlate the ontogeny of non-lymphoid cells and lymphoid cells. Mononuclear phagocytes and reticulum cells were detected in the liver, spleen, yolk sac, bursa, gut, and thymus at about the same time as leukocytes, but earlier than B lymphocytes.To determine whether mononuclear phagocytes and reticulum cells in spleen and liver absorb antigen, we injected embryos intravenously with colloidal carbon and the antigen FITC-Ficoll. In addition, acid phosphatase was used as a marker for phagocytic activity. Reticulum cells in the liver and spleen were functional from the first point of detection, whereas mononuclear phagocytes in the liver and spleen started to absorb antigen a few days after their development.