Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput
Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput
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DOI：
10.1063/1.4902371
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发表时间：
2014-11-01
期刊：
影响因子：
3.2
通讯作者：
Flanagan, Lisa A.
中科院分区：
文献类型：
--
作者：
Simon, Melinda G.;Li, Ying;Flanagan, Lisa A.
Dielectrophoresis （DEP）已被证明是干细胞和祖细胞群体富集的宝贵工具，因为它能够以无标记的方式对细胞进行分类，并且具有生物安全性。然而，DEP分离设备的低通量使研究人员无法进行需要大量细胞的研究，例如细胞移植所需的细胞。我们开发了一种微流体装置，设计用于干细胞和祖细胞群的富集，以150,000细胞/小时的速度对细胞进行分选，相当于我们以前的设备设计的吞吐量提高了一个数量级以上。这一进展，再加上数据显示，depp分选的细胞在培养长达2周的时间内仍能保持其富集和分化能力，提供了足够的吞吐量和细胞数量，可以使用富集的干细胞和祖细胞群体进行更广泛的实验。此外，这里介绍的分选装置易于安装和操作，制造过程简单，使用成本低，使它们更适合在普通生物研究实验室中使用。据我们所知，这项工作代表了第一个使用DEP富集干细胞并在培养中扩增以产生移植规模数量的分化能力细胞的研究。(C) 2014 AIP Publishing LLC。
Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a labelfree manner and its biological safety. However, DEP separation devices have suffered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We developed a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of magnitude. This advancement, coupled with data showing the DEP-sorted cells retain their enrichment and differentiation capacity when expanded in culture for periods of up to 2 weeks, provides sufficient throughput and cell numbers to enable a wider variety of experiments with enriched stem and progenitor cell populations. Furthermore, the sorting devices presented here provide ease of setup and operation, a simple fabrication process, and a low associated cost to use that makes them more amenable for use in common biological research laboratories. To our knowledge, this work represents the first to enrich stem cells and expand them in culture to generate transplantation-scale numbers of differentiation-competent cells using DEP. (C) 2014 AIP Publishing LLC.