2,5-Furandicarboxylic acid production from furfural by sequential biocatalytic reactions
2,5-Furandicarboxylic acid production from furfural by sequential biocatalytic reactions
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DOI：
10.1016/j.jbiosc.2021.03.001
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发表时间：
2021-06-16
影响因子：
2.8
通讯作者：
Kino, Kuniki
中科院分区：
文献类型：
--
作者：
Kawanabe, Kazuki;Aono, Riku;Kino, Kuniki
2，5-呋喃二酸(FDCA)是由生物质衍生的羟甲基呋喃甲醛(HMF)合成的一种有价值的化合物，在聚酰胺、聚酯和聚氨酯的生产中具有很大的潜力作为石油基对苯二甲酸的替代品。然而，从木质纤维生物质中大规模生产HMF的经济策略还没有建立起来。本研究旨在设计一条以木质纤维生物质为原料工业化生产呋喃甲醛的合成路线。该人工途径由依赖于异丙基黄素单核苷酸(PrFMN)的可逆脱羧酶和催化呋喃甲醛氧化的可逆脱羧酶和2-呋喃甲酸的羧化反应组成。在分离菌株Paraburkholderia gerorum KK1中发现了依赖于prFMN的可逆脱羧酶，而从甲氧菌中分离到一种HMF氧化酶。MP688具有较强的呋喃甲醛氧化活性，可作为一种呋喃甲醛氧化酶。利用共表达这些蛋白的Escherichia coif细胞，以及黄素戊烯基转移酶，可以在一锅中通过2-呋喃甲酸合成呋喃西林。(C)2021年，日本生物技术学会。版权所有。
2,5-Furandicarboxylic acid (FDCA) is a valuable compound that can be synthesized from biomass-derived hydroxymethylfurfural (HMF), and holds great potential as a promising replacement for petroleum-based terephthalic acid in the production of polyamides, polyesters, and polyurethanes used universally. However, an economical large-scale production strategy for HMF from lignocellulosic biomass is yet to be established. This study aimed to design a synthetic pathway that can yield FDCA from furfural, whose industrial production from lignocellulosic biomass has already been established. This artificial pathway consists of an oxidase and a prenylated flavin mononucleotide (prFMN)dependent reversible decarboxylase, catalyzing furfural oxidation and carboxylation of 2-furoic acid, respectively. The prFMN-dependent reversible decarboxylase was identified in an isolated strain, Paraburkholderia fungorum KK1, whereas an HMF oxidase from Methylovorus sp. MP688 exhibited furfural oxidation activity and was used as a furfural oxidase. Using Escherichia coif cells coexpressing these proteins, as well as a flavin prenyltransferase, FDCA could be produced from furfural via 2-furoic acid in one pot. (C) 2021, The Society for Biotechnology, Japan. All rights reserved.