Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China
复制标题
华中蛇类隐孢子虫、肝虫和螺旋体的分子鉴定及系统发育分析
DOI：
10.1016/j.ijppaw.2019.10.001
复制
发表时间：
2019-12-01
影响因子：
1.8
通讯作者：
Yu, Xue-Jie
中科院分区：
文献类型：
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作者：
Xiao, Xiao;Qi, Rui;Yu, Xue-Jie
蛇在中国是很受欢迎的食物和传统药物。然而，有关中国蛇的寄生虫和细菌感染的信息很少。我们于2018年6月至10月通过使用寄生虫特异性引物的PCR扩增，调查了中国中部蛇类中选定的人畜共患病因子，包括隐孢子虫，肝虫和迭宫绦虫。PCR扩增和DNA测序结果表明，10.1%（15/149）的蛇隐孢子虫阳性，149例中肝体虫阳性4例，阳性率为2.7%。此外，我们还发现36.9%（55/149）的蛇感染了刺猬迭宫绦虫。每条感染蛇的裂头蚴负担范围为1至26。BLAST和小亚基核糖体RNA（SSU rRNA）基因和60-kDa糖蛋白（gp 60）基因的系统发育分析表明，该虫属于微小隐孢子虫基因型IIdA 15 G1、C. baileyi，C.蛇和肝虫。我们的结论是，密集养殖蛇排泄C。parvum和C.结果表明，我国中部地区野生蛇类普遍感染S. baileyi。erinaceieuropaei，这表明食用烹饪不当的蛇可能对人体健康有风险。
Snakes are popular as food and traditional medicine in China. However, information about parasitic and bacterial infections in snakes from China is scarce. We investigated the prevalence of selected zoonotic agents including Cryptosporidium, Hepatozoon and Spirometra, in snakes in central China from June to October in 2018 by PCR amplification using parasite-specific primers. PCR amplification and DNA sequencing showed that 10.1% (15/149) of snakes were positive for Cryptosporidium spp., while 2.7% (4/149) were positive for Hepatozoon. Additionally, we found 36.9% (55/149) of snakes were infected with Spirometra erinaceieuropaei. The spargana burden per infected snake ranged from 1 to 26. BLAST and phylogenetic analysis of small subunit ribosomal RNA (SSU rRNA) gene and 60-kDa glycoprotein (gp60) gene showed that the parasites belonged to Cryptosporidium parvum genotype IIdA15G1, C. baileyi, C. serpentis and a Hepatozoon species. We conclude that intensively farmed snakes excrete C. parvum and C. baileyi oocysts due to ingestion of infected feeder animals, and that wild snakes in central China were commonly infected with S. erinaceieuropaei, suggesting that eating improperly cooked snakes could be risky to human health.