Estimation of peroxisomal and mitochondrial fatty acid oxidation in rat hepatocytes using tritiated substrates.
Estimation of peroxisomal and mitochondrial fatty acid oxidation in rat hepatocytes using tritiated substrates.
复制标题
使用氚化底物估计大鼠肝细胞中的过氧化物酶体和线粒体脂肪酸氧化。
DOI：
10.1042/bj2790147
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Rognstad,R
中科院分区：
文献类型：
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作者：
Rognstad,R
使用产生 NAD3H 的底物监测过氧化物酶体和线粒体脂肪酸氧化的途径。我使用以下标记物底物：D-[3-3H]3-羟基丁酸用于线粒体 NAD3H 生产，[2-3H]甘油用于胞质 NAD3H 生产，[2-3H]乙酸盐用于测量碳结合 3H，碳结合 3H 也是由商业 9,10-3H 标记脂肪酸的代谢产生的。使用线粒体脂肪酸氧化的特异性抑制剂支持了过氧化物酶体 NAD3H 可以被认为等同于胞质 NAD3H 的假设。该方法涉及确定来自标记物底物和标记脂肪酸的葡萄糖中 3H 的比产率以及碳 4 和 6 上的相对分布。在安妥明治疗大鼠的肝细胞中，过氧化物酶体中开始的棕榈酸盐或油酸盐氧化量与线粒体中开始的相当。
The pathways of peroxisomal and mitochondrial fatty acid oxidation were monitored with the use of substrates which produce NAD3H. I used as marker substrates: D-[3-3H]3-hydroxybutyrate for mitochondrial NAD3H production, [2-3H]glycerol for cytosolic NAD3H production, and [2-3H]acetate to measure carbon-bound 3H which was also generated by the metabolism of the commercial 9,10-3H-labelled fatty acids. The assumption that peroxisomal NAD3H can be considered to be equivalent to cytosolic NAD3H was supported using a specific inhibitor of mitochondrial fatty acid oxidation. The approach involves determination of the specific yields, and the relative distribution on carbons 4 and 6, of 3H in glucose from the marker substrates and the labelled fatty acids. In hepatocytes from clofibrate-treated rats, the amount of palmitate or oleate oxidation which starts in the peroxisomes is comparable with that which starts in the mitochondria.