Comprehensive analysis of chemotactic factors for bone marrow mesenchymal stem cells
Comprehensive analysis of chemotactic factors for bone marrow mesenchymal stem cells
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DOI：
10.1089/scd.2006.0032
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发表时间：
2007-02-01
影响因子：
4
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
--
作者：
Ozaki, Yoshie;Nishimura, Masahiro;Kato, Yukio
为了了解哪些生长因子/细胞因子可以影响间充质干细胞（MSC）向损伤组织的迁移，我们使用微趋化室比较了许多（26）生长因子/细胞因子对兔和人MSC迁移活性的影响。其中，血小板衍生生长因子（PDGF）-BB、PDGF-AB、表皮生长因子（EGF）、HB-EGF、转化生长因子（TGF-α）、胰岛素生长因子（IGF-I）、肝细胞生长因子（HGF）、成纤维细胞生长因子（FGF-2）和凝血酶在适当浓度下一致地增强兔和人MSC的迁移。PDGF-BB对迁移的影响最大。这些因素的各种组合进一步增强了MSC的迁移，而共享共同的细胞表面受体的因素的组合并不诱导添加剂刺激。另一方面，一些组合，包括FGF-2或凝血酶与PDGF-BB的组合，抑制MSC的迁移活性。这些发现表明，生长因子的组合是重要的，以引起最大的趋化作用。诱导MSC迁移的因素也增强了其增殖，表明迁移和增殖可以同时发生。上述因子也有效刺激成纤维细胞的迁移，但凝血酶单独选择性地增强MSC的迁移，表明凝血酶可用于刺激MSC的迁移而不迁移成纤维细胞。
To understand which growth factors/cytokines can affect migration of mesenchymal stem cells (MSCs) to injured tissues, we compared the effects of many (26) growth factors/cytokines on the migration activity of rabbit and human MSCs using a microchemotaxis chamber. Among them, platelet-derived growth factor (PDGF)-BB, PDGF-AB, epidermal growth factor (EGF), HB-EGF, transforming growth factor (TGF-alpha), insulin growth factor (IGF-I), hepatocyte growth factor (HGF), fibroblast growth factor (FGF-2), and thrombin consistently enhanced the migration of rabbit and human MSCs at appropriate concentrations. PDGF-BB showed the greatest effect on migration. Various combinations of these factors further enhanced the migration of MSCs, whereas combinations of factors that shared common cell-surface receptors did not induce the additive stimulation. On the other hand, some combinations, including that of FGF-2 or thrombin with PDGF-BB, suppressed the migration activity of MSCs. These findings suggest that combinations of growth factors are important to eliciting the maximal chemotactic effect. The factors that induced the migration of MSCs also enhanced their proliferation, suggesting that migration and proliferation can take place simultaneously. The above factors were also effective in stimulating the migration of fibroblasts, but thrombin alone selectively enhanced the migration of MSCs, suggesting that thrombin is useful to stimulate migration of MSCs without migration of fibroblasts.