Cloning and characterization of cDNA of the GPI-anchored purple acid phosphatase and its root tissue distribution in Spirodela oligorrhiza.
Cloning and characterization of cDNA of the GPI-anchored purple acid phosphatase and its root tissue distribution in Spirodela oligorrhiza.
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少根紫萍 GPI 锚定紫色酸性磷酸酶 cDNA 的克隆、表征及其根组织分布。
DOI：
10.1034/j.1399-3054.2001.1130212.x
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发表时间：
2001
影响因子：
6.4
通讯作者：
H. Okuyama
中科院分区：
文献类型：
--
作者：
M. Nishikoori;K. Washio;A. Hase;N. Morita;H. Okuyama
采用cDNA文库筛选和5‘末端快速扩增相结合的方法，从低磷条件下生长的少刺紫草植株中获得了糖基磷脂酰肌醇(GPI)锚定的紫色酸性磷酸酶(PAP)的cDNA克隆。寡聚丹参PAP基因的开放阅读框全长1 365bp，编码455个氨基酸的蛋白质。其推导的氨基酸序列与拟南芥和菜豆PAP的同源性分别为82%和80%。氨基酸残基Ala439和另外两个较小的氨基酸残基Asp和Ser被预测为GPI锚定(Omega)位点。在推测的omega位点上游没有二碱性基序，表明PAP是一种细胞壁蛋白。这一假设得到了以下发现的支持：PAP是通过纤维素酶消化细胞壁部分而释放的。推测GPI锚是将PAP运输到细胞壁的信号。-P植物根的免疫组织化学结果表明，PAP优先分布在根最外层的皮层细胞中，而不是在表皮中，这表明在缺磷条件下，PAP在获取无机磷方面起到了作用。Northern杂交分析表明，在-P植物生长过程中，PAP基因的表达增加，PAP基因在-P植物的蛋白质水平和活性水平上也存在着时间依赖性的表达。
A cDNA clone of the glycosylphosphatidylinositol (GPI)-anchored purple acid phosphatase (PAP) has been obtained by a combination of cDNA library screening and 5' rapid amplification of cDNA ends from Spirodela oligorrhiza plants grown under phosphate-deficient (-P) conditions. The open reading frame of the S. oligorrhiza PAP cDNA consists of 1 365 bp encoding a 455 amino acid protein. Its deduced amino acid sequence shows 82 and 80% similarity to Arabidopsis thaliana and Phaseolus vulgaris PAP, respectively. The amino acid residue, Ala439, followed by two more small amino acid residues, Asp and Ser, is predicted to be the GPI-anchoring (omega) site. The absence of a dibasic motif upstream of the putative omega site suggests that the PAP is a cell wall protein. This presumption is supported by the finding that PAP was released by digestion of the cell wall fraction with cellulase. The GPI anchor is speculated to be a signal for transporting PAP to the cell wall. Immunohistochemical results using -P plant roots demonstrate that PAP is preferentially distributed in the outermost cortical cells of roots but not in the epidermis, suggesting its role in acquiring inorganic phosphate under phosphate-deficient conditions. Northern blot analysis using the S. oligorrhiza PAP cDNA as a probe demonstrates that expression of the PAP gene increased during growth of -P plants and this time-dependent occurrence in mRNA levels of the PAP in -P plants was also observed in their protein and activity levels.
曼氏血吸虫：Sm23 是一种跨膜蛋白，还含有糖基磷脂酰肌醇锚。
影响因子：
3.9
作者：
Koster,B;Strand,M
通讯作者：
Strand,M