Sub-5-nm lanthanide-doped ZrO_2@NaYF_4 nanodots as efficient upconverting probes for rapid scanning microscopy and aptamer-mediated bioimaging
Sub-5-nm lanthanide-doped ZrO_2@NaYF_4 nanodots as efficient upconverting probes for rapid scanning microscopy and aptamer-mediated bioimaging
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DOI：
10.1364/ome.5.001759
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发表时间：
2015-08
影响因子：
2.8
通讯作者：
Jing Liu;Nana Li;Ruitao Wu;Yuxiang Zhao;Qiuqiang Zhan;Sailing He
中科院分区：
文献类型：
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作者：
Jing Liu;Nana Li;Ruitao Wu;Yuxiang Zhao;Qiuqiang Zhan;Sailing He
上转换纳米粒子（UCNPs）具有无光漂白和无闪烁发射等优点，是一类很有前途的蛋白质分子成像探针。然而，合成一种超小但明亮的UCNPs仍然具有挑战性。本文设计并实验验证了一种新型高效的亚5 nm上转换纳米点。所提出的均匀UCNDs包括两部分：3.5-nm Ln 3+掺杂的ZrO 2核和0.5-nm NaYF 4壳。所提出的UCNDs的发射强度被测量为比常规的10-nm NaYF 4 UCNPs的发射强度亮10倍以上。在300 kW·cm−2的辐照激发下，掺Er 3 + 15 mol%的UCNDs的发光强度是掺Er 3 + 0.5 mol%的UCNDs的18倍，发光寿命是掺Er 3 + 0.5 mol%的UCNDs的15。使用生物相容性UCND，进行适体介导的蛋白质靶向成像，并表现出高效率。这些超小型、高效的UCNDs在快速扫描和细胞成像方面具有巨大的潜力。
Upconversion nanoparticles (UCNPs) is a class of promising probes widely used in protein molecules imaging due to the no photobleaching and non-blinking emission. However, it is still remained challenging to synthesize a type of ultra-small but bright UCNPs. In this paper, a new class of efficient sub-5-nm upconversion nanodots (UCNDs) was elaborately designed and experimentally demonstrated. The proposed uniform UCNDs comprise two parts: a 3.5-nm Ln3+-doped ZrO2 core and 0.5-nm NaYF4 shell. The emission intensity of the proposed UCNDs was measured to be more than 10 times brighter than that of the conventional 10-nm NaYF4 UCNPs. Under 300 kW·cm−2 irradiance excitation, the 15 mol% Er3+-doped UCNDs exhibit an 18-fold enhanced intensity and one fifth emission lifetime compared to the 0.5 mol% Er3+-doped ones. Using the biocompatible UCNDs, the aptamer-mediated proteins-targeted imaging was performed and demonstrated high efficiency. These ultra-small, efficient UCNDs would have great potentials in rapid scanning and cellular imaging.