Analysis of polymer molecular weight distributions in aqueous two-phase systems.
Analysis of polymer molecular weight distributions in aqueous two-phase systems.
复制标题
水性两相体系中聚合物分子量分布的分析。
DOI：
10.1016/0168-1656(91)90224-j
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发表时间：
1991
影响因子：
4.1
通讯作者：
Kula,MR
中科院分区：
文献类型：
--
作者：
Forciniti,D;Hall,CK;Kula,MR
蛋白质和其他生物材料在含有聚乙二醇和葡聚糖的两个水相之间的分配是两种聚合物的分子量的强函数。尽管两种聚合物都是多分散的（尤其是 Dx），但大多数理论处理仅涉及平均分子量（数量或质量），并假设每种聚合物的分子量分布在两个相中相同。在这项工作中，使用高效凝胶色谱法测量了四种 PEG 储备溶液（4000、6000、10,000 和 20,000）和四种 Dx 储备溶液（10,000、40,000、110,000 和 500,000）的分子量分布。在混合并平衡后，对聚合物溶液形成的相重复测量。 Dx的分子量分布在顶相和底相中不同；两者都与储备溶液不同。尽管我们认为 PEG 的分子量分布在顶相和底相中也不同，但我们无法在仪器的分辨率内确定这一点。
The partitioning of proteins and other biomaterials between two aqueous phases containing polyethyleneglycol and dextran is a strong function of the molecular weight of the two polymers. Although both polymers are polydispersed (especially Dx) most theoretical treatments refer only to the average molecular weight (number or mass) and assume that the molecular weight distribution of each polymer is the same in both phases. In this work the molecular weight distributions of four stock solutions of PEG (4000, 6000, 10,000 and 20,000) and four stock solutions of Dx (10,000, 40,000, 110,000 and 500,000) were measured using High Performance Gel Chromatography. The measurements were repeated on the phases formed by the polymer solutions after they were mixed and allowed to equilibrate. The molecular weight distribution of the Dx differed in the top and bottom phase; both differed from that of the stock solution. Although we believe that the molecular weight distribution for PEG also differs in the top and bottom phases, we were unable to determine this within the resolution of our instruments.