Transformation in restriction-deficient Salmonella typhimurium LT2.
Transformation in restriction-deficient Salmonella typhimurium LT2.
复制标题
限制缺陷型鼠伤寒沙门氏菌 LT2 的转化。
DOI:
10.1099/00221287-135-9-2561
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发表时间:
1989
期刊:
影响因子:
--
通讯作者:
J. Ryu
中科院分区:
文献类型:
--
作者:
S. Tsai;R. J. Hartin;J. Ryu
Stable restriction-deficient, modification-proficient galE (JR501) and F'galE+ (JR502) strains of Salmonella typhimurium were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors which affect transformation efficiency were systematically examined to determine optimum transformation conditions and a simplified method is presented.