Transformation in restriction-deficient Salmonella typhimurium LT2.

Transformation in restriction-deficient Salmonella typhimurium LT2.
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限制缺陷型鼠伤寒沙门氏菌 LT2 的转化。

DOI:
10.1099/00221287-135-9-2561
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发表时间:
1989
期刊:
Journal of general microbiology
影响因子:
--
通讯作者:
J. Ryu
J. Ryu
中科院分区:
--
文献类型:
--
作者:
S. Tsai;R. J. Hartin;J. Ryu

文献摘要

被引文献

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构建了稳定的限制性缺陷、修饰丰富的鼠伤寒沙门氏菌 galE (JR501) 和 F'galE+ (JR502) 菌株,并测试了限制性对质粒 pBR322 转化的影响。系统地研究了影响转化效率的几个因素,以确定最佳转化条件,并提出了一种简化的方法。
Stable restriction-deficient, modification-proficient galE (JR501) and F'galE+ (JR502) strains of Salmonella typhimurium were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors which affect transformation efficiency were systematically examined to determine optimum transformation conditions and a simplified method is presented.