A topoisomerase II-like protein is part of an inducible DNA-binding protein complex that binds 5' of an immunoglobulin promoter.
A topoisomerase II-like protein is part of an inducible DNA-binding protein complex that binds 5' of an immunoglobulin promoter.
复制标题
拓扑异构酶 II 样蛋白是诱导型 DNA 结合蛋白复合物的一部分，可结合免疫球蛋白启动子的 5。
DOI：
10.1093/nar/21.18.4363
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发表时间：
1993
影响因子：
14.9
通讯作者：
Drake,FH
中科院分区：
文献类型：
--
作者：
Webb,CF;Eneff,KL;Drake,FH
We previously Identified a B cell-specific protein-DNA complex 5' of an Immunoglobulin n heavy chain promoter. The sequences to which this protein complex bound were required for induction of immunoglobulin mRNA levels with interleukln-5 + antigen. Further studies identified a second sequence 5' of these regulatory sequences that bound to both the nuclear matrix and to a similar lnterleukin-5 + antigen inducible protein complex. Therefore, we sought to Identify the putative regulatory proteins that comprised this DNA-blnding complex. In this study, we have used anti-topoisomerase II antibodies to demonstrate that one of the proteins found In the lnterleukin-5 + antigen inducible complexes is serologically related to topoisomerase II. To our knowledge, this Is the first report where a topoisomerase II related protein participates In an inducible mobility shifted protein-DNA complex. These data suggest a model In which the enhanced immunoglobulin gene transcription observed after treatment with Interleukln-5 + antigen might be explained by the Induction of a protein complex that acts to relieve torsional stress along the gene.
