Pro-apoptotic versus anti-apoptotic properties of dietary resveratrol on tumoral and normal cardiac cells
Pro-apoptotic versus anti-apoptotic properties of dietary resveratrol on tumoral and normal cardiac cells
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DOI：
10.1007/s12263-011-0232-z
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发表时间：
2011-05-01
影响因子：
3.5
通讯作者：
Latruffe, Norbert
中科院分区：
文献类型：
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作者：
Baarine, Mauhamad;Thandapilly, Sijo Joseph;Latruffe, Norbert
白藜芦醇是一种天然的膳食多酚，存在于葡萄皮、红酒和各种其他食品中。白藜芦醇已被证明是不同恶性肿瘤的有效化学预防药物。它还被证明可以防止血管改变，如动脉粥样硬化和炎症相关事件。有鉴于此，我们研究了白藜芦醇对小鼠肿瘤心房肌细胞系(HL-1NB)的抗增殖和促凋亡作用。这些作用与在正常新生小鼠心肌细胞上发现的结果进行了比较。白藜芦醇(5、30和/或100mU)作用于HL-1NB细胞和新生心肌细胞不同的培养时间(24、48和/或72h)。用显微镜和流式细胞仪检测白藜芦醇的作用。白藜芦醇处理后，观察到肿瘤心脏HL1-NB细胞的生长受到强烈抑制，并伴随着细胞黏附的丧失。这种细胞增殖停滞与细胞的凋亡过程有关，表现为经Hoechst 33342染色后核碎裂和/或浓缩的细胞百分比增加(凋亡细胞的特征)，以及亚G1期细胞的存在。白藜芦醇对正常心肌细胞无细胞毒作用，对去甲肾上腺素诱导的心肌细胞凋亡有保护作用。总之，目前的数据表明，白藜芦醇(1)诱导肿瘤心脏HL1-NB细胞凋亡，(2)不诱导正常心肌细胞死亡，(3)阻止去甲肾上腺素诱导的正常心肌细胞凋亡。
Resveratrol is a natural dietary polyphenol found in grape skin, red wine, and various other food products. Resveratrol has proved to be an effective chemopreventive agent for different malignant tumors. It has also been shown to prevent vascular alterations such as atherosclerosis and inflammatory-associated events. In view of these observations, we investigated the anti-proliferative and pro-apoptotic activities of resveratrol on a tumoral cardiac cell line (HL-1 NB) derived from mouse tumoral atrial cardiac myocytes. These effects were compared with those found on normal neonatal mouse cardiomyocytes. HL-1 NB cells and neonatal cardiomyocytes were treated with resveratrol (5, 30, and/or 100 mu M) for different times of culture (24, 48, and/or 72 h). Resveratrol effects were determined by various microscopical and flow cytometric methods. After resveratrol treatment, a strong inhibition of tumoral cardiac HL1-NB cell growth associated with a loss of cell adhesion was observed. This cell proliferation arrest was associated with an apoptotic process revealed by an increased percentage of cells with fragmented and/or condensed nuclei (characteristic of apoptotic cells) identified after staining with Hoechst 33342 and by the presence of cells in subG1. At the opposite, on normal cardiomyocytes, no cytotoxic effects of resveratrol were observed, and a protective effect of resveratrol against norepinephrine-induced apoptosis was found on normal cardiomyocytes. Altogether, the present data demonstrate that resveratrol (1) induces apoptosis of tumoral cardiac HL1-NB cells, (2) does not induce cell death on normal cardiomyocytes, and (3) prevents norepinephrine-induced apoptosis on normal cardiomyocytes.