A mitochondrial genome phylogeny of the Neuropterida (lace-wings, alderflies and snakeflies) and their relationship to the other holometabolous insect orders
A mitochondrial genome phylogeny of the Neuropterida (lace-wings, alderflies and snakeflies) and their relationship to the other holometabolous insect orders
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DOI：
10.1111/j.1463-6409.2009.00392.x
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发表时间：
2009-11-01
影响因子：
2.5
通讯作者：
Whiting, Michael F.
中科院分区：
文献类型：
--
作者：
Cameron, Stephen L.;Sullivan, Jaron;Whiting, Michael F.
卡梅伦，S.L.，沙利文，J.，宋，H.，米勒，K.B. 和怀廷，M.F. (2009)。脉翅目昆虫（花边翅目、赤蝇和蛇蝇）的线粒体基因组系统发育及其与其他全变态昆虫目的关系。 - Zoologica Scripta, 38, 575-590。我们提出了线粒体 (mt) 基因组系统发育学，推断脉翅目（草翅目、赤蝇和骆驼蝇）内以及脉翅目与其他全变态昆虫目之间的关系。对Sialis hamata（巨翅目：Sialidae）、Ditaxis latistyla（神经翅目：Mantispidae）、Mongoloraphidia Harmandi（Raphidioptera：Raphidiidae）、Macrogyrus oblongus（鞘翅目：Gyrinidae）、Rhopaea magnicornis（鞘翅目：Scarabaeidae）和Mordella atrata的整个mt基因组进行了测序（鞘翅目：Mordellidae）并在系统发育分析中与其他全变态目的代表进行比较。此外，我们测试了系统发育推断对四种分析方法的敏感性：RNA 基因的包含与排除、手动与算法比对、排除可变基因区域的任意与算法方法以及每种方法如何与系统发育推断方法相互作用（简约与贝叶斯推断）。其中，系统发育推断方法对序间关系的影响最大。贝叶斯分析推断的拓扑结构与基于形态学的神经翅目关系假设基本一致，神经翅目是一种单系神经翅目，其姊妹类群是鞘翅目。相比之下，简约分析未能支持单系脉翅目，因为针翅目是整个全变态纲（不包括膜翅目）的姐妹群，而脉翅目+大翅目是双翅目的姐妹群，这种关系以前从未根据分子或形态学数据集提出过。分析方法之间的这些差异是由于昆虫 mt 基因组中发现的高位点率异质性所致，该异质性可以通过贝叶斯方法正确建模，但会导致简约下的人为关系。经过适当的分析，这里提供的 mt 基因组数据集是支持传统的、基于形态学的对三个神经翅目及其与鞘翅目分组之间关系的解释的首批分子数据之一。
Cameron, S. L., Sullivan, J., Song, H., Miller, K. B. & Whiting, M. F. (2009). A mitochondrial genome phylogeny of the Neuropterida (lace-wings, alderflies and snakeflies) and their relationship to the other holometabolous insect orders. - Zoologica Scripta, 38, 575-590.We present a mitochondrial (mt) genome phylogeny inferring relationships within Neuropterida (lacewings, alderflies and camel flies) and between Neuropterida and other holometabolous insect orders. Whole mt genomes were sequenced for Sialis hamata (Megaloptera: Sialidae), Ditaxis latistyla (Neuroptera: Mantispidae), Mongoloraphidia harmandi (Raphidioptera: Raphidiidae), Macrogyrus oblongus (Coleoptera: Gyrinidae), Rhopaea magnicornis (Coleoptera: Scarabaeidae), and Mordella atrata (Coleoptera: Mordellidae) and compared against representatives of other holometabolous orders in phylogenetic analyses. Additionally, we test the sensitivity of phylogenetic inferences to four analytical approaches: inclusion vs. exclusion of RNA genes, manual vs. algorithmic alignments, arbitrary vs. algorithmic approaches to excluding variable gene regions and how each approach interacts with phylogenetic inference methods (parsimony vs. Bayesian inference). Of these factors, phylogenetic inference method had the most influence on interordinal relationships. Bayesian analyses inferred topologies largely congruent with morphologically-based hypotheses of neuropterid relationships, a monophyletic Neuropterida whose sister group is Coleoptera. In contrast, parsimony analyses failed to support a monophyletic Neuropterida as Raphidioptera was the sister group of the entire Holometabola excluding Hymenoptera, and Neuroptera + Megaloptera is the sister group of Diptera, a relationship which has not previously been proposed based on either molecular or morphological data sets. These differences between analytical methods are due to the high among site rate heterogeneity found in insect mt genomes which is properly modelled by Bayesian methods but results in artifactual relationships under parsimony. Properly analysed, the mt genomic data set presented here is among the first molecular data to support traditional, morphology-based interpretations of relationships between the three neuropterid orders and their grouping with Coleoptera.