Genome-Wide Analysis of MIKCC-Type MADS Box Genes in Grapevine
Genome-Wide Analysis of MIKCC-Type MADS Box Genes in Grapevine
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DOI：
10.1104/pp.108.131052
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发表时间：
2009-01-01
期刊：
影响因子：
7.4
通讯作者：
Jose Carmona, Maria
中科院分区：
文献类型：
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作者：
Diaz-Riquelme, Jose;Lijavetzky, Diego;Jose Carmona, Maria
MIKCC型MADS盒基因编码的转录因子在植物生长发育中起着重要作用。对葡萄藤（葡萄属vinifera）基因组的分析揭示了多达38个MIKCC型基因。我们在这里报告一个完整的分析，这个基因家族的系统发育关系与同源基因在其他测序的双子叶植物基因组，其基因组位置，基因结构和表达。葡萄藤基因与拟南芥和白杨的对应基因聚在13个亚科中。缺乏最近的全基因组重复葡萄允许分配基因多样化的过程中观察到的每个亚科内的祖先多倍体化事件之前的分歧，这三个物种或以后的重复事件在每个谱系。MIKCC型基因在营养和生殖器官以及花和卷须发育过程中的表达谱显示特定亚科的保守表达域，但也反映葡萄藤发育的特征。在潜伏芽和花发育过程中的表达分析揭示了以前在其他植物系统中描述的共同特征，以及在开花过渡期间一些亚科成员可能的新角色。葡萄藤中MIKCC型基因的分析有助于了解每个亚科内基因多样性的起源，并为功能分析提供基础，以揭示这些MADS盒基因在葡萄藤发育中的作用。
MIKCC-type MADS box genes encode transcription factors that play crucial roles in plant growth and development. Analysis of the grapevine (Vitis vinifera) genome revealed up to 38 MIKCC-type genes. We report here a complete analysis of this gene family regarding their phylogenetic relationships with homologous genes identified in other sequenced dicot genomes, their genome location, and gene structure and expression. The grapevine genes cluster in 13 subfamilies with their Arabidopsis (Arabidopsis thaliana) and poplar (Populus trichocarpa) counterparts. The lack of recent whole genome duplications in grapevine allows assigning the gene diversification processes observed within each subfamily either to an ancestral polyploidization event predating the divergence of those three species or to later duplication events within each lineage. Expression profiles of MIKCC-type genes in vegetative and reproductive organs as well as during flower and tendril development show conserved expression domains for specific subfamilies but also reflect characteristic features of grapevine development. Expression analyses in latent buds and during flower development reveal common features previously described in other plant systems as well as possible new roles for members of some subfamilies during flowering transition. The analysis of MIKCC-type genes in grapevine helps in understanding the origin of gene diversification within each subfamily and provides the basis for functional analyses to uncover the role of these MADS box genes in grapevine development.