Shikonin potentiates paclitaxel antitumor efficacy in esophageal cancer cells via the apoptotic pathway
Shikonin potentiates paclitaxel antitumor efficacy in esophageal cancer cells via the apoptotic pathway
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DOI：
10.3892/ol.2019.10662
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发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Jie
中科院分区：
文献类型：
--
作者：
Du, Wenzhen;Hao, Xiaohong;Liu, Jie
紫草素是一种天然的萘醌色素，可以抑制多种癌细胞的生长。紫杉醇是一种化疗药物，用于治疗各种类型的实体瘤癌症。然而，获得性紫杉醇耐药导致治疗失败，以及随之而来的转移和复发。本研究的目的是探讨紫草素是否能提高食管癌细胞对紫杉醇的敏感性，并阐明其作用机制。采用MTT法、细胞周期分析、Annexin-V凋亡分析、Western blotting和逆转录-定量聚合酶链反应（RT-PCR）等方法，研究两种药物对食管癌细胞株KYSE 270和KYSE 150的生物学效应。结果表明，紫草素能显著增强紫杉醇对细胞生长的抑制作用（P
Shikonin is a natural naphthoquinone pigment that can suppress the growth of a number of cancer cell types. Paclitaxel is an antineoplastic chemotherapy drug, which is used for the treatment of various types of solid tumor cancer. However, acquired paclitaxel resistance results in the failure of therapy, and consequent metastasis and relapse. The aim of the present study was to investigate whether shikonin can sensitize esophageal cancer cells to paclitaxel-treatment and to elucidate the underlying mechanisms. The biological effects of these two agents on esophageal cancer cell lines KYSE270 and KYSE150 were investigated by MTT assay, cell cycle analysis, Annexin-V apoptosis assay, western blotting and reverse transcription-quantitative polymerase chain reaction. The results demonstrated that shikonin could significantly increase the cell growth inhibition effect induced by paclitaxel in the examined cell lines (P