MOLECULAR-CLONING OF A COMPLEMENTARY-DNA ENCODING HUMAN MACROPHAGE-SPECIFIC COLONY-STIMULATING FACTOR (CSF-1)
MOLECULAR-CLONING OF A COMPLEMENTARY-DNA ENCODING HUMAN MACROPHAGE-SPECIFIC COLONY-STIMULATING FACTOR (CSF-1)
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DOI：
10.1126/science.2996129
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
MARK, DF
中科院分区：
文献类型：
--
作者：
KAWASAKI, ES;LADNER, MB;MARK, DF
分离编码人巨噬细胞特异性集落刺激因子（CSF-1）的互补DNA （cDNA）克隆。一个cDNA克隆编码一个包含224个氨基酸的成熟多肽和一个包含32个氨基酸的推定先导。该cDNA克隆在Okayama-Berg表达载体上，通过特异性放射受体测定、巨噬细胞骨髓集落形成和抗体中和来确定COS细胞中生物活性CSF-1的合成。大多数cDNA分离物含有改变阅读框的内含子序列的一部分，导致翻译的突然终止；这些cDNA在COS细胞中无活性。CSF-1似乎是由一个单拷贝基因编码的，但它的表达导致了几个信使RNA物种的合成，大小从1.5到4.5千碱基不等。
Complementary DNA (cDNA) clones encoding human macrophage-specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids. This cDNA, which was cloned in the Okayama-Berg expression vector, specifies the synthesis of biologically active CSF-1 in COS cells, as determined by a specific radioreceptor assay, macrophage bone marrow colony formation, and antibody neutralization. Most of the cDNA isolates contain part of an intron sequence that changes the reading frame, resulting in an abrupt termination of translation; these cDNA's were inactive in COS cells. The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases.