BENDING PATTERNS OF CHLAMYDOMONAS FLAGELLA .4. MUTANTS WITH DEFECTS IN INNER AND OUTER DYNEIN ARMS INDICATE DIFFERENCES IN DYNEIN ARM FUNCTION
BENDING PATTERNS OF CHLAMYDOMONAS FLAGELLA .4. MUTANTS WITH DEFECTS IN INNER AND OUTER DYNEIN ARMS INDICATE DIFFERENCES IN DYNEIN ARM FUNCTION
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DOI：
10.1002/cm.970080110
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发表时间：
1987-01-01
影响因子：
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通讯作者：
KAMIYA, R
中科院分区：
文献类型：
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作者：
BROKAW, CJ;KAMIYA, R
外动力蛋白臂缺陷或缺陷的突变体都显示出拍频的主要减少约一半的正常值，这些突变体中的一些显示出与剪切振幅减少和拍频的额外减少相关的滑动速度的额外减少，以及正常前向模式弯曲模式的其他更小的修改。新的突变体（ida98，pf30），这似乎是缺乏一个子集的内部动力蛋白武器显示滑动速度的减少，主要是与剪切振幅的减少，只有一个小的减少拍频。内动力蛋白臂突变体和外动力蛋白臂突变体之间运动表型的这些差异表明，内动力蛋白臂和外动力蛋白臂可能具有不同的功能。滑动速度相对较大的下降与内臂的部分损失是一致的，与早期的观察pf23，一个nonmotile突变缺乏内臂，这表明内臂可能有一个重要的功能，在运动。产生反向模式弯曲模式的能力保留在一些内部或外部动力蛋白臂突变体，但似乎是减少在那些突变体，显示出减少的剪切振幅的前向模式弯曲模式。
Mutants with outer dynein arm defects or deficiencies all show a major reduction in beat frequency to about half the normal value; some of these mutants show an additional decrease in sliding velocity associated with reduced shear amplitude and an additional reduction in beat frequency, as well as other more minor modifications of the normal forward mode bending pattern. New mutants (ida98, pf30), which appear to be deficient in a subset of inner dynein arms show a reduction in sliding velocity that is primarily associated with a reduction in shear amplitude, with only a small reduction in beat frequency. These differences in motility phenotype between inner and outer dynein arm mutants suggest that inner and outer dynein arms may have distinct functions. The relatively large decrease in sliding velocity associated with partial loss of inner arms is consistent with earlier observations on pf23, a nonmotile mutant lacking inner arms, suggesting that inner arms may have an essential function in motility. The ability to generate reverse mode bending patterns is retained in some inner or outer dynein arm mutants, but appears to be decreased in those mutants which show reduced shear amplitude for the forward mode bending pattern.