ALTERATION OF MEMBRANE-FUSION AS A CAUSE OF ACUTE-PANCREATITIS IN THE RAT
ALTERATION OF MEMBRANE-FUSION AS A CAUSE OF ACUTE-PANCREATITIS IN THE RAT
复制标题
DOI：
10.1007/bf01391745
复制
发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
KERN, HF
中科院分区：
文献类型：
--
作者：
ADLER, G;ROHR, G;KERN, HF
超大剂量雨蛙肽诱导大鼠急性水肿性胰腺炎。主胰管插管不能防止水肿的形成，但显示胰血流量几乎完全减少。使用冷冻断裂技术和薄切片电子显微镜，最早的结构变化，观察在酶原颗粒和质膜的膜。酶原颗粒相互融合导致细胞质内形成大的膜结合空泡。这些和单个酶原颗粒与基底外侧质膜融合，将其内容物释放到间隙中。研究结果表明，在特异性的细胞内膜融合过程中诱导的超最大剂量的胰腺促分泌素，最终导致胰腺的自身消化的严重变化。
Infusion of supramaximal doses of cerulein induces acute edematous pancreatitis in the rat. Cannulation of the main pancreatic duct does not prevent the formation of the edema but reveals an almost complete reduction of pancreatic flow. Using freeze-fracture techniques and thin-section electron microscopy, earliest structural alterations were observed at membranes of zymogen granules and the plasma membrane. Fusion of zymogen granules among each other leads to formation of large membrane-bound vacuoles within the cytoplasm. These and individual zymogen granules fuse with the basolateral plasma membrane, discharging their content into the interstitial space. The findings indicate severe changes in the specificity of the intracellular membrane fusion process induced by supramaximal doses of a pancreatic secretagogue, which finally result in autodigestion of the pancreas.