HIV-1 subtype B′ dictates the AIDS epidemic among paid blood donors in the Henan and Hubei provinces of China
HIV-1 subtype B′ dictates the AIDS epidemic among paid blood donors in the Henan and Hubei provinces of China
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DOI：
10.1097/00002030-200311210-00015
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发表时间：
2003-11-21
期刊：
影响因子：
3.8
通讯作者：
Chen, ZW
中科院分区：
文献类型：
--
作者：
Su, R;Liu, L;Chen, ZW
目的：为了解河南、湖北两省有偿献血者（PBD）中HIV-1感染株的遗传背景，采用聚合酶链反应（PCR）技术，从外周血单个核细胞中扩增HIV-1 gag p17基因片段和部分5 '端半基因组或全长基因组。结果：共检测62份样本，其中59份（95.2%）来自PBD。在这59个PBD中，45个来自河南的14个不同的地理位置，而其余14个来自湖北的5个位置。共获得62个HIV-1 gag p17片段，3个5 '端半基因组序列和1个全基因组序列。这些序列的系统发育分析表明，来自河南和湖北的PBD，尽管地理上遥远，窝藏一组遗传上密切相关的HIV-1 B '毒株。结论：河南、湖北两省PBD人群艾滋病流行主要是由HIV-1 B '亚型引起的，而我国其他地区吸毒人群中主要流行的是HIV-1 B'亚型重组株CRF-07和CRF-08。我们的报告首次揭示了河南和湖北最主要的HIV-1流行毒株的完整基因组背景，这对于该地区设计和开发有效的艾滋病疫苗至关重要。(C)2003年利平科特威廉姆斯威尔金斯。
Objective: To investigate the genetic background of HIV-1 strains among infected paid blood donors (PBD) in the Henan and Hubei provinces of China.Methods: The polymerase chain reaction was used to amplify HIV-1 gag p17 fragments and some 5'-half or full-length viral genomes from peripheral blood mononuclear cells. The HIV-1 sequences obtained were analysed using phylogenetic and recombinant approaches.Results: Among the total of 62 samples studied, 59 (95.2%) came from PBD. Of those 59 PBD, 45 were collected from 14 different geographical locations in Henan, whereas the remaining 14 were from five locations in Hubei. A total of 62 HIV-1 gag p17 fragments, three 5'-half and one full HIV-1 genome sequences were obtained. Phylogenetic analysis of these sequences suggests that PBD from Henan and Hubei, despite being geographically distant, harbored a group of genetically closely related HIV-1 B'strains. No recombinant forms have been detected in this cohort.Conclusion: Our data indicate that the AIDS epidemic among PBD in Henan and Hubei is caused largely by HIV-1 subtype B', which contrasts greatly with the CB' recombinant strains CRF-07 and CRF-08 currently dominating among the drug abusers in other regions of China. Our report has revealed the first complete genome background of the most dominant circulating HIV-1 strain in Henan and Hubei, which is essential for the design and development of an effective AIDS vaccine for the region. (C) 2003 Lippincott Williams Wilkins.