Discrimination of Cys from Hcy by an Iridium(III) Complex Based on Time-Dependent Luminescence
Discrimination of Cys from Hcy by an Iridium(III) Complex Based on Time-Dependent Luminescence
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DOI：
10.1021/om400032d
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发表时间：
2013-10
期刊：
影响因子：
2.8
通讯作者：
Hui-li Chen;Xiaokai Li;Rui-Rong Bai;Yanbo Wu;Ying Fan;Jian-Bing Chao
中科院分区：
文献类型：
--
作者：
Hui-li Chen;Xiaokai Li;Rui-Rong Bai;Yanbo Wu;Ying Fan;Jian-Bing Chao
报道了一种半胱氨酸（Cys）的磷光探针Ir（ppy）_2Cl（DMF）（Ir_2）。该识别基于Ir 2-Cys的时间依赖性发射。重要的是，该探针可以快速区分Cys和同型半胱氨酸（Hcy）。探针Ir 2在30 min内显示出高选择性的72 nm的发光红移至Cys，并具有明显的发射颜色从绿色变为橙子。30 min后，发射带逐渐蓝移，发射从橙子变为绿色。TDDFT结合CV研究表明，不同的发射曲线归因于Ir 2与Cys随时间的不同结合模式。该探针可用于半水溶液中半胱氨酸的测定。
A phosphorescent probe, Ir(ppy)2Cl(DMF) (Ir2), for cysteine (Cys) is reported. The recognition is based on the time-dependent emission of Ir2-Cys. Importantly, this probe can discriminate Cys rapidly from homocysteine (Hcy). Probe Ir2 displays a highly selective 72 nm of luminescent red-shift toward Cys within 30 min with an obvious emissive color change from green to orange. After 30 min, the emission band blue shifts gradually with an emissive change from orange to green. TDDFT combined with CV studies indicates that the different emission profile is ascribed to the different binding modes of Ir2 to Cys with time. The probe can be used to determine Cys in semiaqueous solution.