microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells
microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells
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DOI：
10.3892/or.2013.2452
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发表时间：
2013-07-01
期刊：
影响因子：
4.2
通讯作者：
Xie, Liping
中科院分区：
文献类型：
--
作者：
Mao, Yeqing;Chen, Hong;Xie, Liping
MicroRNAs(MiRNAs)是一种小的非编码RNA，已成为大量基因的关键调控因子。本研究旨在探索miR-330在人前列腺癌DU145和PC3细胞中的新的生物学功能。我们证实miR-330表达下调，并且与特异性蛋白1(Sp1)的表达呈负相关。通过转染化学合成的miR-330模拟物过表达miR-330可诱导Sp1蛋白表达水平降低，同时显著抑制细胞的迁移和侵袭能力。此外，Sp1基因敲除实验也出现了类似的现象。最后，荧光素酶报告实验证实Sp1是miR-330的直接靶标。这些发现表明miR-330在前列腺癌中起到了抗转移miRNA的作用。
microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.