Proteomic characterization of Jurkat T leukemic cells after dopamine stimulation: A model of circulating dopamine-sensitive cells
Proteomic characterization of Jurkat T leukemic cells after dopamine stimulation: A model of circulating dopamine-sensitive cells
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DOI：
10.1016/j.biochi.2011.01.015
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发表时间：
2011-05-01
期刊：
影响因子：
3.9
通讯作者：
Fasano, Mauro
中科院分区：
文献类型：
--
作者：
Alberio, Tiziana;Anchieri, Claudia;Fasano, Mauro
T细胞是循环中的多巴胺敏感细胞，在外周水平上可能反映了多巴胺能神经元中发生的生化修饰。人类CD_4~+T细胞白血病Jurkat细胞系已被广泛应用，并被认为是研究T细胞信号和凋亡的合适细胞模型。在这里，我们将它们描述为循环的多巴胺敏感细胞的模型，并通过分析细胞蛋白质组的变化来描述它们对多巴胺挑战的反应。Jurkat细胞既表达D_1和D_2样多巴胺受体，也表达膜和囊泡转运蛋白，但缺乏04受体和酪氨酸羟基酶的表达。饱和、无毒、无氧化剂的50 AM多巴胺激发诱导相互作用的伴侣网络上调，从而证实T细胞是帕金森病的生物标记物发现来源。在多巴胺治疗后，β-肌动蛋白和14-3-亚型分布模式的重塑一直被观察到。总体而言，这里观察到的多巴胺特异性改变可能代表着外周水平的多巴胺反应的生物传感器。(C)2011年爱思唯尔·马森公司。版权所有。
T-cells are circulating dopamine-sensitive cells and may mirror, at the peripheral level, biochemical modifications occurring in dopaminergic neurons. The human CD4+ T leukemic Jurkat cell line has been thoroughly used and characterized as a suitable cell model to investigate T-cell signaling and apoptosis. Here, we describe their characterization as a model of circulating dopamine-sensitive cells and their response to a dopamine challenge by analyzing changes in the cell proteome. Jurkat cells express both D1- and D2-like dopamine receptors and both membrane and vesicular transporters, while they lack 04 receptor and tyrosine hydroxylase expression. A saturating, non-toxic, non-oxidant 50 AM dopamine challenge induces the upregulation of an interacting chaperone network known to protect specifically dopaminergic neurons, thus validating T-cells as a biomarker discovery source in Parkinson's disease. Remodeling of the distribution pattern of beta-actin and 14-3- isoforms is consistently observed upon dopamine treatment. As a whole, dopamine-specific alterations here observed might represent a biosensor for dopamine response at the peripheral level. (C) 2011 Elsevier Masson SAS. All rights reserved.