Classification and identification of Actinobacillus actinomycetemcomitans and haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations.
Classification and identification of Actinobacillus actinomycetemcomitans and haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations.
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通过聚类分析和脱氧核糖核酸杂交对伴放线放线杆菌和嗜血嗜血杆菌进行分类和鉴定。
DOI：
10.1111/j.1600-0765.1982.tb01180.x
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发表时间：
1982
影响因子：
3.5
通讯作者：
Holt,SC
中科院分区：
文献类型：
--
作者：
Tanner,AC;Visconti,RA;Socransky,SS;Holt,SC
将从晚期牙周袋中分离出的15个革兰氏阴性糖溶杆菌与伴放线Arlinohacillusactinomycetemcomitans、嗜沫嗜血杆菌和副嗜沫嗜血杆菌的典型菌株和参考菌株进行比较。生物的表型特征被用于聚类分析和多维标度。从所有菌株中提取脱氧核糖核酸（DNA），并测定鸟嘌呤加胞嘧啶含量的摩尔百分比。对各菌种的参考菌株与口腔分离株进行DNA/DNA同源性实验。5株来自牙周病病变的菌株、2株来自Papillon-Lefevre牙周病病变的菌株和4株来自成人牙周炎的菌株与A.聚类分析和DNA/DNA同源性分析。只有一个H。paraphrophilus isolate，H.嗜热嗜热菌和嗜副嗜热嗜热菌在生化特性和DNA/DNA同源性上存在重叠，不能区分为两个不同的种。从牙周病病变中分离的两株菌株、从成人牙周炎部位分离的两株菌株和三株口腔参考菌株彼此非常相似，并且与H.利用表型特征的嗜阿弗提菌型菌株。未发现过氧化氢分解是A的一致特征。从牙周袋中分离的放线菌。
Fifteen gram‐negative, saccharolytic rods isolated from advanced periodonlal pockeis were compared to type and reference strains of Arlinohacillus actinomycetemcomitans, Haemaphilus aphrophilus, and reference strains of Haemophilus paraphrophilus. Phenotypic features of organisms were used in cluster analysis and multidimensional scaling. Deoxyribonucleic acid (DNA) was extracted from all strains and moles percent guanine plus cytosine content determined. DNA/DNA homology experiments were performed between reference strains of each species and oral isolates. Five strains from periodontosis lesions, two strains from Papillon‐Lefevre periodontal lesions, and four strains from periodontitis pockeis of adults were similar to A. actinomycetemcomitans by cluster analyses and by DNA/DNA homologies. Except for a single H. paraphrophilus isolate, the H. aphrophilus and H, paraphrophilus isolates could not be distinguished as two distinct species as there was overlap in biochemical characteristics and DNA/DNA homology. Two strains isolated from periodontosis lesions, two strains from adult periodontilis sites, and three oral reference strains were very similar to each other and to the H. aphrtiphilus type strain using phenotypic features. Hydrogen peroxide decomposition was not found to be a consistent feature of A. actinomycetemcumitans strains isolated from periodontal pockets.