Molecular genetic analysis of easily accessible breast tumour DNA, purified from tissue left over from hormone receptor measurement
Molecular genetic analysis of easily accessible breast tumour DNA, purified from tissue left over from hormone receptor measurement
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DOI：
10.1111/j.1699-0463.1998.tb01359.x
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发表时间：
1998-03-01
期刊：
影响因子：
2.8
通讯作者：
Kruse, TA
中科院分区：
文献类型：
--
作者：
Hansen, LL;Andersen, J;Kruse, TA
为了建立一个来自原发性乳腺癌患者的正常和肿瘤DNA的大面板，我们寻找一个容易获得的、高质量的乳腺肿瘤DNA来源。在医院进行常规激素受体分析后，剩余的颗粒中含有来自肿瘤组织的细胞核。我们在2年1/4的时间里收集了670个微球，并分离了大量的DNA（平均每个微球400 μ g）。为了控制肿瘤DNA的质量，我们分析了41粒和匹配的正常DNA的杂合性缺失（LOH），并在17号染色体上有11个微卫星标记。这条染色体很好地描述了夸耀癌。LOH是一种灵敏的方法，需要高质量和纯净的肿瘤DNA。正常DNA的污染会模糊检测结果。我们发现高LOH率在33 - 74%之间，这与其他报道一致，因此推荐这种丰富的乳腺肿瘤DNA来源进行分子生物学分析。
In order to establish a large panel of normal and tumour DNA from primary breast cancer patients, we looked for a source of easily accessible, good quality breast tumour DNA. Following routine hormone receptor analysis at the hospital the leftover pellets contained the nuclei from the tumour tissue. We collected 670 pellets over a period of 2 1/4 years and isolated a large amount of DNA (on average 400 mu g per pellet). To control the quality of this tumour DNA, we analysed 41 pellets and matching normal DNA for loss of heterozygosity (LOH), with 11 microsatellite markers along chromosome 17. This chromosome is well described for boast cancer. LOH is a sensitive method, requiring good quality and pure tumour DNA. Contamination with normal DNA will blur the results. We found a high rate of LOH, ranging from 33 to 74%, which is in agreement with other reports, and therefore recommend this rich source of breast tumour DNA for molecular biological analysis.