Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices
Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices
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DOI：
10.1007/s00216-007-1496-4
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Kitamori, Takehiko
中科院分区：
文献类型：
--
作者：
Goto, Makiko;Tsukahara, Takehiko;Kitamori, Takehiko
开发了一种新型微器件，该器件具有用于微芯片中细胞粘附的微米和纳米尺度图案化表面。该表面具有通过电子束光刻和金属溅射制造的金属图案和由烷醇的自组装单层组成的化学图案。金属图案化表面具有宽300 nm、高150 nm的小的金条图案，并且形貌和化学性质都可以控制。小鼠成纤维细胞NIH/3 T3细胞在图案化表面上培养，并沿沿着伸长。这些细胞识别图案的大小和图案上的化学性质，尽管图案比它们小得多。在微芯片中的新鲜培养基流动下有令人满意的细胞生长。图案化表面和微芯片的结合为细胞提供了一个新的生长环境，并将促进许多细胞实验。
A novel microdevice which had a micro- and nanometer-scale patterned surface for cell adhesion in a microchip was developed. The surface had a metal pattern fabricated by electron-beam lithography and metal sputtering and a chemical pattern consisting of a self-assembled monolayer of alkanethiol. The metal patterned surface had a gold stripe pattern which was as small as 300 nm wide and 150 nm high and both topography and chemical properties could be controlled. Mouse fibroblast NIH/3T3 cells were cultured on the patterned surface and elongated along the gold stripes. These cells recognized the size of the pattern and the chemical properties on the pattern though it was much smaller than they were. There was satisfactory cell growth under fresh medium flow in the microchip. The combination of the patterned surface and the microchip provides cells with a novel environment for their growth and will facilitate many cellular experiments.