Nuclear and cytoplasmic localization of neural stem cell microRNAs
Nuclear and cytoplasmic localization of neural stem cell microRNAs
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DOI：
10.1261/rna.2006511
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发表时间：
2011-04-01
期刊：
影响因子：
4.5
通讯作者：
Perkins, Diana O.
中科院分区：
文献类型：
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作者：
Jeffries, Clark D.;Fried, Howard M.;Perkins, Diana O.
虽然通常认为在细胞质中起作用，但在细胞核中发现了许多microRNA（miRNAs），可能在基因调控中起作用。在这里，我们报告说，事实上，所有人类miRNA的相当大一部分存在于神经干细胞的细胞核中。此外，这些miRNA的子集在这些细胞的细胞核中比在细胞质中一致地显示出更高的标准化等级，如用RT-qPCR技术鉴定并通过微阵列分析证实的。同样，其他miRNA显示出更高的细胞质标准化等级。在针对373个miRNAs的六个测定中，将三个样品分为细胞核和细胞质部分。从100个最高表达的miRNA中，确定细胞核和细胞质浓度的标准评分。其中，21种miRNAs的所有三个核标准评分均高于所有三个细胞质评分;同样，31种miRNAs的细胞质评分始终较高。随机浓度会导致每组只有五个。值得注意的是，如果一种miRNA在一个区室中具有高标准评分，那么具有相同5'种子和某些相似3'末端模式的其他miRNA也以相同的方式获得高评分。也就是说，除了种子序列之外，3'序列相似性标准鉴定具有一致高的细胞核或细胞质表达的成熟miRNA家族。
Although generally regarded as functional in the cytoplasm, a number of microRNAs ( miRNAs) have been found in the nucleus, possibly with a role in gene regulation. Here we report that, in fact, a substantial fraction of all human miRNAs are present in the nucleus of neural stem cells. Further, subsets of these miRNAs display consistently higher standardized rank in the nucleus than in the cytoplasm of these cells, as identified with an RT-qPCR technology and confirmed by microarray analysis. Likewise, other miRNAs display higher cytoplasmic standardized ranks. Three samples were partitioned into nuclear and cytoplasmic fractions in six assays for 373 miRNAs. From the 100 most highly expressed miRNAs, standard scores of nuclear and cytoplasmic concentrations were determined. Among those, 21 miRNAs had all three nuclear standard scores higher than all three cytoplasmic scores; likewise, 31 miRNAs had consistently higher cytoplasmic scores. Random concentrations would result in only five in each set. Remarkably, if one miRNA has a high standard score in a compartment, then other miRNAs having the same 5' seeds and certain similar 3' end patterns are also highly scored in the same way. That is, in addition to the seed sequence, 3' sequence similarity criteria identify families of mature miRNAs with consistently high nuclear or cytoplasmic expression.