Lovastatin inhibits adipogenesis and prevents osteonecrosis in steroid-treated rabbits
Lovastatin inhibits adipogenesis and prevents osteonecrosis in steroid-treated rabbits
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洛伐他汀抑制类固醇治疗兔子的脂肪生成并预防骨坏死
DOI：
10.1016/j.jbspin.2007.12.008
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发表时间：
2008-12-01
期刊：
影响因子：
4.2
通讯作者：
Cheng Jingqiu
中科院分区：
文献类型：
--
作者：
Kang Pengde;Pei Fuxing;Cheng Jingqiu
目的：为探讨洛伐他汀对激素性骨坏死的重要机制--脂肪生成的抑制作用及对激素性骨坏死的预防作用，采用54只家兔，一次性肌肉注射醋酸甲泼尼龙（MPSL）20 mg/kg。然后将受试者分为两组：I组给予洛伐他汀，II组给予安慰剂。另16只家兔注射生理盐水（PS）作为对照组（III组）。进行血液学检查以测定血清脂质水平。注射前及注射后1、2、4、12周分别取股骨和肱骨进行组织病理学检查，观察骨坏死情况。结果：注射后1、2、4周，Ⅱ组血脂水平明显高于Ⅰ组和Ⅲ组。骨坏死的发生率在组II（69%）中显著高于组I（36%）和组III（0%）。病理学检查显示，I组的脂肪生成和骨死亡较II组轻。骨髓中脂肪细胞的大小和面积在I组中明显减小（47.5 +/- 1.3亩，25%）和第三组（41.7 +/- 1.6 μ m，12%）比第II组（59.8 ± 6.3 μ m，51%）（P < 0.001）。结论：洛伐他汀可通过抑制脂肪生成来预防激素性骨坏死的发生。对洛伐他汀在临床上的有效性仍需进一步评价。(C)2008年，Elsevier Masson SAS。All rights reserved.
Objectives: To investigate in vivo effects of lovastatin on inhibition of adipogenesis, an important mechanism of steroid-induced osteonecrosis, and on prevention of occurrence of steroid-induced osteonecrosis in rabbits.Methods: Fifty-four rabbits were intramuscularly injected once with 20 mg/kg of methylprednisolone acetate (MPSL). Then, they were divided into two groups: lovastatin was given in Group I and placebo was given in Group II. And another 16 rabbits were injected with physiologic saline (PS) as a control (Group III). Hematological examination was performed for serum lipid levels. Both the femora and the humeri were histopathologically examined for the presence of osteonecrosis before the injection and 1, 2, 4 and 12 weeks after the injection.Results: The serum lipid levels were significantly higher in Group II than in Groups I and III 1, 2 and 4 weeks after the injection. The incidence of osteonecrosis was significantly higher in Group II (69%) than in Group I (36%) and Group III (0%). Pathological examination showed less serious adipogenesis and bone death in Group I than in Group II. The size and area of the fat cells in the bone marrow were significantly smaller in Group I (47.5 +/- 1.3 mu m, 25%) and Group III (41.7 +/- 1.6 mu m, 12%) than in Group II (59.8 +/- 6.3 mu m, 51%) (P < 0.001).Conclusion: Lovastatin can prevent development of steroid-induced osteonecrosis in rabbits by inhibiting adipogenesis. Future evaluation on the effectiveness of lovastatin in the clinical practice is still necessary. (C) 2008 Elsevier Masson SAS. All rights reserved.