Amyloid proteins : methods and protocols
Amyloid proteins : methods and protocols
复制标题
淀粉样蛋白：方法和方案
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
M. Gasset
中科院分区：
文献类型：
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作者：
E. Sigurdsson;M. Calero;M. Gasset
第一部分.体外试验：用于组装和毒性试验的无聚集体低分子量淀粉样蛋白-b的制备Gal Bitan和大卫B。快速光化学交联Sabrina S. Vollers，大卫B. Teplow和Gal Bitan体外制备淀粉样蛋白-b和α-突触核蛋白Hilal A的前纤维中间体。Lashuel和Dolors Grillo-Bosch重组Tau蛋白的纯化和体外阿尔茨海默配对螺旋丝的制备Stefan Barghorn，Jacek Biernat和Eckhard Mandelkow蛋白质错误折叠和聚集的循环扩增Paula Saa，Joaquin Castilla和Claudio Soto淀粉样结构O的X射线衍射研究作者：Sumner Makin，Louise C. Serpell分子电子显微镜技术研究蛋白质纤维形成机制。Lashuel和Joseph S.壁延时原子力显微镜在淀粉样纤维组装和低聚中间体表征中的应用Claire Goldsbury和Janelle绿色傅里叶变换红外光谱和圆二色性光谱用于淀粉样蛋白研究Miguel Calero和Maria Gasset准弹性光散射用于蛋白组装研究Aleksey Lomakin，大卫B.特普罗和乔治B。Tau构象和聚集的Benedek固有荧光检测Martin von卑尔根，Li Li，and Eckhard Mandelkow通过质谱法定量测量纤维形成Andrew D.第二部分.人血管平滑肌细胞的分离和培养。Thormodsson和Ingvar H. Olafsson小鼠脑血管细胞作为脑淀粉样血管病的细胞培养模型：从小鼠脑中分离平滑肌和内皮细胞。Jung和Efrat Levy从转染细胞的条件培养基中纯化人野生型或变体胱抑素C Frances Prelli，Monika Pawlik，Blas Frangione和Efrat Levy朊病毒在细胞培养中的增殖Sylvain Lehmann第III部分。体内相关试验淀粉样蛋白增强因子的制备和增殖从富含淀粉样蛋白的人类组织中纯化淀粉样蛋白AA亚种Gunilla T.从富含淀粉样蛋白的人类组织中纯化甲状腺素运载蛋白和甲状腺素运载蛋白片段。蛋白质分析和淀粉样蛋白-b定量前的组织处理放大图片作者：施密特，姜颖.尼克松和保罗M.马修斯酶联免疫吸附法测定淀粉样蛋白-b水平放大图片作者：施密特尼克松和保罗M.小鼠脑淀粉样蛋白b的马修斯组织学染色痒病小鼠模型：接种、临床评分和组织学技术。Meeker，Xuemin Ye，and Richard I.淀粉样蛋白-b肽的鲤鱼放射性标记Miguel Calero和Jorge Ghiso用多光子显微镜对小鼠大脑中淀粉样蛋白-b沉积物进行体内成像Jesse Skoch、Gregory A.作者：Stephen T. Kajdasz，布拉德利T. Hyman和Brian J. Bacskai转基因小鼠中淀粉样蛋白斑块的磁共振成像Youssef Zaim Wadghiri，Einar M. Sigurdsson，托马斯Wisniewski，和丹尼尔H.特恩布尔指数
Part I. In Vitro Assays Preparation of Aggregate-Free, Low Molecular Weight Amyloid-b for Assembly and Toxicity Assays Gal Bitan and David B. Teplow Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking Sabrina S. Vollers, David B. Teplow, and Gal Bitan In Vitro Preparation of Prefibrillar Intermediates of Amyloid-b and a-Synuclein Hilal A. Lashuel and Dolors Grillo-Bosch Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro Stefan Barghorn, Jacek Biernat, and Eckhard Mandelkow Cyclic Amplification of Protein Misfolding and Aggregation Paula Saa, Joaquin Castilla, and Claudio Soto X-Ray Diffraction Studies of Amyloid Structure O. Sumner Makin and Louise C. Serpell Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis Hilal A. Lashuel and Joseph S. Wall Time-Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates Claire Goldsbury and Janelle Green Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies Miguel Calero and Maria Gasset Quasielastic Light Scattering for Protein Assembly Studies Aleksey Lomakin, David B. Teplow, and George B. Benedek Intrinsic Fluorescent Detection of Tau Conformation and Aggregation Martin von Bergen, Li Li, and Eckhard Mandelkow Quantitative Measurement of Fibrillogenesis by Mass Spectrometry Andrew D. Miranker Part II. Cell Culture Assays Isolation and Culturing of Human Vascular Smooth Muscle Cells Finnbogi R. Thormodsson and Ingvar H. Olafsson Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells From Mouse Brain Sonia S. Jung and Efrat Levy Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells Frances Prelli, Monika Pawlik, Blas Frangione, and Efrat Levy Prion Propagation in Cell Culture Sylvain Lehmann Part III. In Vivo-Related Assays Preparation and Propagation of Amyloid-Enhancing Factor Robert Kisilevsky Purification of Amyloid Protein AA Subspecies From Amyloid-Rich Human Tissues Gunilla T. Westermark and Per Westermark Purification of Transthyretin and Transthyretin Fragments From Amyloid-Rich Human Tissues Per Westermark and Gunilla T. Westermark Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens Fernando Goni and Gloria Gallo Tissue Processing Prior to Protein Analysis and Amyloid-b Quantitation Stephen D. Schmidt, Ying Jiang, Ralph A. Nixon, and Paul M. Mathews ELISA Method for Measurement of Amyloid-b Levels Stephen D. Schmidt, Ralph A. Nixon, and Paul M. Mathews Histological Staining of Amyloid-b in Mouse Brains Einar M. Sigurdsson The Mouse Model for Scrapie: Inoculation, Clinical Scoring, and Histopathological Techniques Harry C. Meeker, Xuemin Ye, and Richard I. Carp Radiolabeling of Amyloid-b Peptides Miguel Calero and Jorge Ghiso In Vivo Imaging of Amyloid-b Deposits in Mouse Brain With Multiphoton Microscopy Jesse Skoch, Gregory A. Hickey, Stephen T. Kajdasz, Bradley T. Hyman, and Brian J. Bacskai Magnetic Resonance Imaging of Amyloid Plaques in Transgenic Mice Youssef Zaim Wadghiri, Einar M. Sigurdsson, Thomas Wisniewski, and Daniel H. Turnbull Index