Nanotube surface triggers increased chondrocyte extracellular matrix production
Nanotube surface triggers increased chondrocyte extracellular matrix production
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DOI：
10.1016/j.msec.2010.01.013
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发表时间：
2010-05-10
影响因子：
7.9
通讯作者：
Jin, Sungho
中科院分区：
文献类型：
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作者：
Brammer, Karla S.;Oh, Seunghan;Jin, Sungho
我们已经改变了TiO 2纳米管的直径从30到100 nm的阳极氧化，并研究了在体外牛软骨软骨细胞（BCC）的响应不同的纳米尺度。垂直排列的二氧化钛纳米管结构的纳米形貌触发增强的整体生产的细胞外基质（ECM）成分在平坦的钛控制表面没有基于纳米结构的表面形貌。最初的SEM观察显示，BCC在纳米管基底上产生致密的ECM原纤维，这在平坦的Ti上是缺乏的。生物化学检查证实，培养基中的糖胺聚糖（GAG）分泌也在纳米管基底上上调，在70 nm直径的纳米管上达到最高产量，在平坦的Ti上GAG分泌放大100%。PCR分析显示，聚集蛋白聚糖和II型胶原蛋白的转录水平增加的纳米管表面与70 nm直径的纳米管表现出最高的相对水平的两个软骨形成的转录水平。这项研究表明，TiO 2纳米管结构在70 nm的直径制度，已经是一种骨整合的生物材料，有显着的和有利的影响，软骨细胞的细胞外基质的生产，可能有令人鼓舞的影响，在软骨/骨界面骨软骨治疗利用纳米技术的材料应用。(C)2010 Elsevier B. V.保留所有权利。
We have altered TiO2 nanotube diameters from 30 to 100 nm by anodization and investigated the in vitro bovine cartilage chondrocyte (BCC) response to the different nanoscale dimensions. The nanotopography of the vertically aligned TiO2 nanotube structures triggered enhanced overall production of extracellular matrix (ECM) components over flat Ti control surfaces without a nanostructure-based surface topography. Initial SEM observations revealed that BCCs produced dense ECM fibrils on nanotubular substrates, which were lacking on flat Ti. Biochemical examination confirmed that glycosaminoglycan (GAG) secretion in the culture media was also up-regulated on nanotube substrates, reaching the highest production on the 70 nm diameter nanotubes with 100% amplification in GAG secretion over flat Ti. PCR analysis revealed that aggrecan and collagen type II transcription levels were increased on nanotube surfaces with the 70 nm diameter nanotubes exhibiting the highest relative levels of both chondrogenic transcription levels. This study demonstrates that TiO2 nanotube structures in the 70 nm diameter regime, already being an osseo-integrating biomaterial, have significant and favorable effects on the extracellular matrix production of chondrocytes that could have encouraging implications for material applications in cartilage/bone interface osteochondral treatments utilizing nanotechnology. (C) 2010 Elsevier B.V. All rights reserved.