DNA-Probing for Genes Coding for Denitrification, N2-Fixation and Nitrification in Bacteria Isolated from Different Soils
DNA-Probing for Genes Coding for Denitrification, N2-Fixation and Nitrification in Bacteria Isolated from Different Soils
复制标题
DNA 探测不同土壤中分离细菌的反硝化、固氮和硝化基因编码
DOI：
10.1515/znc-1998-1-214
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Bothe
中科院分区：
文献类型：
--
作者：
K. Kloos;Ursula M. Hüsgen;H. Bothe
摘要采用基因探针集落杂交技术，对科隆地区4种土壤不同层分离的细菌进行反硝化、硝化和固氮分离。在测试的土壤中，反硝化细菌在分离的总体中所占的百分比为3 - 8%（在一个例外情况下为15%），因此很小。反硝化细菌在表层（深度~ 5 cm）富集，仅在深度25 cm处少量存在。硝酸盐显然不能决定这些土壤中反硝化细菌的分布。不同土层的潜在反硝化活性与dna探测评估的分离株分布模式一致。在植物根部或根部的细菌总数和反硝化分离物的数量明显高于邻近植物的块状无根土壤。分离出的好氧固氮菌的百分比在0 ~ 3%之间，这些细菌主要来自上层5 cm层。一小部分分离株的杂交探针编码了欧洲亚硝化单胞菌氨单加氧酶的一个亚基。研究表明，dna探针可以提供反硝化细菌和固氮细菌在不同土壤及其层间相对分布的有用信息。
Abstract Bacteria isolated from different layers of four soils of the Cologne area were analyzed for denitrifying, nitrifying and N2-fixing isolates by colony hybridization using gene probes. In the soils tested, the percentage of denitrifying bacteria among the total population isolated was 3 -8 % (in one case exceptionally 15%) and thus small. Denitrifying bacteria were particularly enriched in the upper layer (depth ~ 5 cm) and were present only in low amounts at 25 cm depth in two gleysol soils. Nitrate apparently did not determine the distribution of denitrifying bacteria in these soils. The potential denitrification activity of different soil layers coincided with the distribution pattern of isolates assessed by DNA-probing. The total number of bacteria and of denitrifying isolates was considerably higher in or at the roots of plants than in the bulk, root-free soil adjacent to the plants. The percentage of the isolated aerobic N2-fixing bacteria varied between 0-3 % , and these bacteria could be isolated mainly from the upper 5 cm layer. A small portion of the isolates hybridized with the probe coding for part of one subunit of ammonia monooxygenase from Nitrosomonas europaea. The investigation showed that DNA-probing can provide useful information about the relative distribution of denitrifying and N2-fixing bacteria in different soils and their layers.