In vivo model for visualizing flap microcirculation of ischemia-reperfusion
In vivo model for visualizing flap microcirculation of ischemia-reperfusion
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DOI：
10.1002/micr.10050
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发表时间：
2002-01-01
期刊：
影响因子：
2.1
通讯作者：
Harii, K
中科院分区：
文献类型：
--
作者：
Ichioka, S;Minh, TC;Harii, K
由于缺血-再灌注损伤仍然是显微外科重建中的主要问题，因此仍然需要改进实验模型。我们开发了一种模型，允许直接可视化皮瓣微循环在小鼠活体显微镜技术。在小鼠背部安装一个新设计的皮褶室，用活体显微镜观察微循环。岛状皮瓣，滋养的旋髂深动脉，提高植入后的腔室，使可视化的微循环岛状皮瓣。通过夹闭蒂部使岛状皮瓣暴露于全脑缺血，然后释放夹具以允许再灌注。观察缺血再灌注引起的各种微循环反应。该模型准确地模拟了再造手术中的临床情况，并成功地实现了活体皮瓣微循环的慢性可视化。
Since ischemia-reperfusion injury continues to be a major problem in reconstructive microsurgery, improvement of experimental models is still desirable. We developed a model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. A newly designed skinfold chamber was installed on the dorsum of mice, and microcirculation was inspected with an intravital microscope. An island flap, nourished by the deep circumflex iliac arteries, was elevated after implantation of the chamber, allowing visualization of the microcirculation in the island flap. The island flap was exposed to global ischemia by clamping the pedicles, and the clamps were then released to allow reperfusion. Various microcirculatory responses induced by ischemia-reperfusion were visualized. This model accurately simulated the clinical situation in reconstructive surgery and successfully realized chronic visualization of the flap microcirculation in vivo.