Effect of mass dihydroartemisinin-piperaquine administration in southern Mozambique on the carriage of molecular markers of antimalarial resistance.
Effect of mass dihydroartemisinin-piperaquine administration in southern Mozambique on the carriage of molecular markers of antimalarial resistance.
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DOI：
10.1371/journal.pone.0240174
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Mayor A
中科院分区：
文献类型：
--
作者：
Gupta H;Galatas B;Chidimatembue A;Huijben S;Cisteró P;Matambisso G;Nhamussua L;Simone W;Bassat Q;Ménard D;Ringwald P;Rabinovich NR;Alonso PL;Saúte F;Aide P;Mayor A
大规模给药可迅速减轻恶性疟原虫(PF)的负担。然而，人们仍然担心它在选择抗疟疾耐药性方面的贡献。在莫桑比克南部马古德地区连续两年实施两轮双氢青蒿素-哌喹(DHAP)每月两轮丙二醛治疗前(n=99)和实施后(n=112)，我们用Sanger测序和实时定量聚合酶链式反应(Real-Time-PCR)测定了与抗疟疾耐药性相关的分子标记(K13、pfpm2、pfmdr1和pfcrt)的比例。在所分析的PF分离株中未观察到与青蒿素耐药性相关的K13基因多态性。与哌喹耐药相关的多拷贝pfpm2的PF分离株的比例在丙二醛治疗前(4.9%)和治疗后(3.4%；p=1.000)相似。在丙二醛治疗前和治疗后的两组中，pfcrt和pfmdr1基因既没有突变，也没有携带pfmdr1多拷贝的PF分离株的比例在统计学上没有显著差异(p>；0.05)。这项研究没有任何证据表明，在莫桑比克南部，在干预开始时抗疟药标志物缺乏或较低的地方，丙二醛感染DHAP后，抗疟疾耐药性分子标志物的频率增加。
Mass drug administration (MDA) can rapidly reduce the burden of Plasmodium falciparum (Pf). However, concerns remain about its contribution to select for antimalarial drug resistance. We used Sanger sequencing and real-time PCR to determine the proportion of molecular markers associated with antimalarial resistance (k13, pfpm2, pfmdr1 and pfcrt) in Pf isolates collected before (n = 99) and after (n = 112) the implementation of two monthly MDA rounds with dihydroartemisinin–piperaquine (DHAp) for two consecutive years in Magude district of Southern Mozambique. None of the k13 polymorphisms associated with artemisinin resistance were observed in the Pf isolates analyzed. The proportion of Pf isolates with multiple copies of pfpm2, an amplification associated with piperaquine resistance, was similar in pre- (4.9%) and post-MDA groups (3.4%; p = 1.000). No statistically significant differences were observed between pre- and post-MDA groups in the proportion of Pf isolates neither with mutations in pfcrt and pfmdr1 genes, nor with the carriage of pfmdr1 multiple copies (p>0.05). This study does not show any evidence of increased frequency of molecular makers of antimalarial resistance after MDA with DHAp in southern Mozambique where markers of antimalarial resistance were absent or low at the beginning of the intervention.
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与柬埔寨恶性疟原虫疟疾双氢青蒿素-哌喹失败相关的遗传标记：基因型-表型关联研究。
DOI：
10.1016/s1473-3099(16)30409-1
发表时间：
2017-03
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Amato R;Lim P;Miotto O;Amaratunga C;Dek D;Pearson RD;Almagro-Garcia J;Neal AT;Sreng S;Suon S;Drury E;Jyothi D;Stalker J;Kwiatkowski DP;Fairhurst RM
通讯作者：
Fairhurst RM
影响因子：
--
作者：
Landier J;Kajeechiwa L;Thwin MM;Parker DM;Chaumeau V;Wiladphaingern J;Imwong M;Miotto O;Patumrat K;Duanguppama J;Cerqueira D;Malleret B;Rénia L;Nosten S;von Seidlein L;Ling C;Proux S;Corbel V;Simpson JA;Dondorp AM;White NJ;Nosten FH
通讯作者：
Nosten FH
影响因子：
6.4
作者：
Dhingra SK;Redhi D;Combrinck JM;Yeo T;Okombo J;Henrich PP;Cowell AN;Gupta P;Stegman ML;Hoke JM;Cooper RA;Winzeler E;Mok S;Egan TJ;Fidock DA
通讯作者：
Fidock DA
影响因子：
11.8
作者：
Gupta H;Macete E;Bulo H;Salvador C;Warsame M;Carvalho E;Ménard D;Ringwald P;Bassat Q;Enosse S;Mayor A
通讯作者：
Mayor A
影响因子：
9.3
作者：
Amaratunga, Chanaki;Andrianaranjaka, Voahangy Hanitriniaina;Woodrow, Charles
通讯作者：
Woodrow, Charles