Bactericidal Effect of Various Laser Irradiation Systems on Enterococcus faecalis Biofilms in Dentinal Tubules: A Confocal Laser Scanning Microscopy Study
Bactericidal Effect of Various Laser Irradiation Systems on Enterococcus faecalis Biofilms in Dentinal Tubules: A Confocal Laser Scanning Microscopy Study
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各种激光照射系统对牙本质小管内粪肠球菌生物膜的杀菌作用：共焦激光扫描显微镜研究
DOI：
10.1089/pho.2017.4430
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发表时间：
2018
影响因子：
--
通讯作者：
He Wenxi
中科院分区：
文献类型：
--
作者：
Wang Xiaoli;Cheng Xiaogang;Liu Xin;Wang Zhihua;Wang Juan;Guo Cuihong;Zhang Yaqing;He Wenxi
目的：通过建立牙本质感染模型和共聚焦激光扫描显微镜（CLSM）技术，比较不同激光照射系统对牙本质小管粪肠球菌生物膜的杀菌效果。将粪便离心到牙本质小管中，培养3周，然后接受如下1分钟和3分钟处理：（A）5.25%次氯酸钠（NaOCl）冲洗，（B）Nd：YAG激光照射，（C）二极管激光照射，（D）Nd：雅普激光照射，（E）Er，Cr：YSGG激光激活的NaOCl冲洗，和（F）Er：YAG激光激活的NaOCl冲洗。结果：对于每个组，细菌减少随着治疗时间的增加而增加（p< 0.05）。Er、Cr：YSGG和Er：YAG激光对NaOCl的杀菌作用有显著增强作用（p< 0.05）。结论：在本实验条件下，Er：YAG + NaOCl和Er，Cr：YSGG + NaOCl的根管消毒效果最好，是一种有潜力的根管消毒方法。
Objective:The aim of this study was to compare the bactericidal effect of various laser irradiation systems onEnterococcus faecalisbiofilms in dentinal tubules by using a novel dentin infection model and confocal laser scanning microscopy (CLSM).Background data:Laser-activated irrigations have been proposed as an adjuvant to conventional protocols of root canal treatment to enhance the smear layer removal, which is a promising protocol for root canal disinfection.Materials and methods:E. faecaliswere centrifuged into the dentinal tubules, cultured for 3 weeks, and then received 1- and 3-min treatments as follows: (A) 5.25% sodium hypochlorite (NaOCl) irrigation, (B) Nd:YAG laser irradiation, (C) diode laser irradiation, (D) Nd:YAP laser irradiation, (E) Er,Cr:YSGG laser-activated NaOCl irrigation, and (F) Er:YAG laser-activated NaOCl irrigation. Bacterial reductions were assessed by CLSM using a LIVE/DEAD®bacterial viability stain method.Results:For each group, the bacterial reduction increased as the treatment time increased (p< 0.05). The Er,Cr:YSGG and Er:YAG laser significantly enhanced the bactericidal effect of NaOCl (p< 0.05). Under the conditions of the same treatment time, bacterial reductions were presented in the descending order of Er:YAG + NaOCl, Er,Cr:YSGG + NaOCl > Nd:YAP > Nd:YAG, diode > NaOCl.Conclusions:Under the conditions of present study, treatments of Er:YAG + NaOCl and Er,Cr:YSGG + NaOCl presented the strongest bactericidal effect among the tested protocols and are potential protocols for root canal disinfection.