Real time monitoring of the cell viability during treatment with tumor-targeted toxins and saponins using impedance measurement.
Real time monitoring of the cell viability during treatment with tumor-targeted toxins and saponins using impedance measurement.
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DOI：
10.1016/j.bios.2012.03.024
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发表时间：
2012-05
影响因子：
12.6
通讯作者：
M. Thakur;K. Mergel;A. Weng;Stefanie Frech;R. Gilabert-Oriol;D. Bachran;M. Melzig;H. Fuchs
中科院分区：
文献类型：
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作者：
M. Thakur;K. Mergel;A. Weng;Stefanie Frech;R. Gilabert-Oriol;D. Bachran;M. Melzig;H. Fuchs
这项工作描述了应用阻抗为基础的测量的真实的时间评价靶向肿瘤治疗细胞培养（HeLa细胞）。我们使用了一种在细胞和小鼠中已经建立的治疗方法。其中，用名为SE的基于表皮生长因子的靶向毒素和称为皂苷的特定植物糖苷的组合来治疗肿瘤细胞。在本研究中，将HeLa细胞以不同数量接种到整合到96孔板底部的叉指电极结构上。在存在和不存在皂苷SpnS-1（分离自肥皂草根）的情况下用SE处理细胞。阻抗与细胞的活力直接相关。正如已知终点测量所预期的，观察到毒性的浓度依赖性增强;然而，通过阻抗测量，我们首次能够追踪联合治疗期间细胞死亡的时间变化。这大大增加了对皂苷增强靶毒素毒性的初始细胞机制的理解，并表明真实的时间监测优于终点测定。该方法劳动强度低，无标签，易于监测每个时间点的效果。
This work describes the application of an impedance-based measurement for the real time evaluation of targeted tumor therapies in cell culture (HeLa cells). We used a treatment procedure that is well established in cells and mice. Therein, tumor cells are treated with a combination of an epidermal growth factor-based targeted toxin named SE and particular plant glycosides called saponins. In the present study HeLa cells were seeded in different numbers onto interdigitated electrode structures integrated into the bottom of a 96 well plate. The cells were treated with SE in the presence and absence of the saponin SpnS-1 (isolated from Saponaria officinalis roots). The impedance was directly correlated with the viability of the cells. As expected from known end point measurements, a concentration dependent enhancement of toxicity was observed; however, with the impedance measurement we were for the first time able to trace the temporal changes of cell death during the combination treatment. This substantially added to the understanding of initial cellular mechanisms in the augmentation of the toxicity of targeted toxins by saponins and indicated the superiority of real time monitoring over end point assays. The method is less labor intensive and label-free with ease of monitoring the effects at each time point.