Amperometric glucose biosensor based on boron-doped carbon nanotubes modified electrode
Amperometric glucose biosensor based on boron-doped carbon nanotubes modified electrode
复制标题
基于硼掺杂碳纳米管修饰电极的电流型葡萄糖生物传感器
DOI：
10.1016/j.talanta.2008.04.023
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发表时间：
2008-08-15
期刊：
影响因子：
6.1
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Chen, Xiaoli;Chen, Jinhua;Yao, Shouzhuo
由于其独特的物理化学性质，掺杂碳纳米管现在在生物分析应用中是极具吸引力和重要的纳米材料。在本文中，掺硼碳纳米管（BCNT）被用于电流生物传感器。研究发现，由于硼掺杂引起的大量边缘位点和位于缺陷位点的富氧基团，BCNTs修饰的玻碳（GC）电极对过氧化氢氧化的电催化活性远高于未掺杂的CNTs修饰电极。选择葡萄糖氧化酶（GOD）作为模型酶，通过将GOD包埋在聚（邻氨基苯酚）膜中将其固定在BCNTs修饰的玻碳电极上。通过电化学方法研究了传感器的性能。该生物传感器在最佳电位+0.60 V、pH 7.0下表现出灵敏度高(171.2 nA mM(-1))、检测下限低(3.6 μM)、响应时间短(6 s以内)、良好的抗干扰能力和良好的稳定性等特点。表观米氏常数 (K-m(app)) 为 15.19 mM。还评估了酶电极对全血分析的适用性。 (C) 2008 Elsevier B.V. 保留所有权利。
Doped carbon nanotubes are now extremely attractive and important nanomaterials in bioanalytical applications due to their unique physicochemical properties. In this paper, the boron-doped carbon nanotubes (BCNTs) were used in amperometric biosensors. It has been found that the electrocatalytic activity of the BCNTs modified glassy carbon (GC) electrode toward the oxidation of hydrogen peroxide is much higher than that of the un-doped CNTs modified electrode due to the large amount of edge sites and oxygen-rich groups located at the defective sites induced by boron doping. Glucose oxidase (GOD) was selected as the model enzyme and immobilized on the BCNTs modified glassy carbon electrode by entrapping GOD into poly(o-aminophenol) film. The performance of the sensor was investigated by electrochemical methods. At an optimum potential of +0.60 V and pH 7.0, the biosensor exhibits good characteristics, such as high sensitivity (171.2 nA mM(-1)), low detection limit(3.6 mu M), short response time (within 6 s), satisfactory anti-interference ability and good stability. The apparent Michaelis-Menten constant (K-m(app)) is 15.19 mM. The applicability to the whole blood analysis of the enzyme electrode was also evaluated. (C) 2008 Elsevier B.V. All rights reserved.