Contrasting cellular responses in Schistosoma haematobium infected and exposed individuals from areas of high and low transmission in Zimbabwe
Contrasting cellular responses in Schistosoma haematobium infected and exposed individuals from areas of high and low transmission in Zimbabwe
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DOI：
10.1016/s0165-2478(03)00088-9
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发表时间：
2003-09-08
影响因子：
4.4
通讯作者：
Hagan, P
中科院分区：
文献类型：
--
作者：
Mduluza, T;Ndhlovu, PD;Hagan, P
这项研究比较了来自两个地区的个体的细胞因子谱，这两个地区具有不同的埃及血吸虫传播模式。一个是高传输区（HT），另一个是低传输区（LT）。埃及血吸虫对刺激的外周血单核细胞（PBMC）进行细胞免疫反应观察，这些细胞是在治疗前、治疗后12个月和18个月收集的。刺激是与寄生虫和卵抗原和有丝分裂原，phaetohemaglutinin（PHA）。观察PBMC增殖和在5天孵育期内产生的细胞因子的概况。两个不同区域在所有测量类别（IL-4、IL-5、IL-10和IFN-γ）的增殖和细胞因子产生水平上均显示出显著差异。来自LT区域的个体的PBMC对两种抗原刺激剂具有高水平的增殖但低细胞因子产生，而来自HT区域的个体的PBMC显示低水平的增殖但高细胞因子产生水平。在治疗前，在HT区域未排泄卵小体的个体比感染个体对刺激物的增殖水平更高。然而，治疗后再次感染的个体表现出高水平的增殖。在治疗前，感染和未感染组分别显示出低和相似的IL-4：IFN-γ、IL-5：IFN-γ和IL-10：IFN-γ的比率，而IFN-γ在感染个体中较高。治疗后，未再感染者的IL-4、IL-5和IL-10水平较高，感染者的IFN-γ水平较高。Th 1样反应占主导地位，在感染与Th 2样反应占主导地位的治疗后和未感染的个人。结果表明，细胞因子的平衡部分地决定了对S.血吸虫感染(C)2003 Elsevier Science B. V.保留所有权利。
The study compared cytokine profiles of individuals from two areas with different transmission patterns for Schistosoma haematobium. One area was a high transmission (HT) while the other was a low transmission (LT) area for S. haematobium. Observations on cellular immune responses were made on stimulated peripheral blood mononuclear cells (PBMC), which were collected pre-treatment, then at 12 and 18 months post treatment. Stimulation was with schistosome worm and egg antigens and a mitogen, phaetohaemaglutinin (PHA). Observations were made on PBMC proliferation and the profiles of cytokine produced over a 5-day incubation period. The two distinct areas showed significant differences on both levels of proliferation and cytokine production for all the measured classes (IL-4, IL-5, IL-10 and IFN-gamma). PBMC from individuals from the LT area had high levels of proliferation but low cytokine production to both antigen stimulants while PBMC from individuals from the HT area showed low levels of proliferation but high cytokine production levels. Prior to treatment, individuals not excreting schistosome ova in the HT area had higher levels of proliferation to the stimulants, than the infected individuals. However, after treatment re-infected individuals showed high levels of proliferation. Before treatment, both infected and uninfected groups showed low and similar ratios, respectively, of IL-4:IFN-gamma, IL-5:IFN-gamma and IL-10:IFN-gamma, while IFN-gamma was high in the infected individuals. After treatment the non re-infected had higher levels of IL-4, IL-5 and IL-10, with the infected having high levels of IFN-gamma. Th1-like response dominated during infection with the Th2-like responses dominating post treatment and in uninfected individuals. The results indicated that the cytokine balance determines, in part, susceptibility or resistance to S. haematobium infection. (C) 2003 Elsevier Science B.V. All rights reserved.