Mitochondrial hydrogen peroxide production as determined by the pyridine nucleotide pool and its redox state
Mitochondrial hydrogen peroxide production as determined by the pyridine nucleotide pool and its redox state
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DOI：
10.1016/j.bbabio.2012.03.033
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发表时间：
2012-10-01
影响因子：
4.3
通讯作者：
Vinogradov, Andrei D.
中科院分区：
文献类型：
--
作者：
Kareyeva, Alexandra V.;Grivennikova, Vera G.;Vinogradov, Andrei D.
膜结合牛心呼吸复合物I的NADH支持的超氧化物/过氧化氢产生速率。可溶性猪心二氢硫辛酰胺脱氢酶（DLDH），并通过伴随的操作，这些酶在大鼠心脏线粒体基质中被测量为吡啶核苷酸库及其氧化还原状态的函数。每一个活动都表现出对核苷酸池浓度的非平凡依赖性。测定其半数最大容量所需的NAD（+）/NADH比率。约一半的总NADH支持的H2 O2生产的透性线粒体在刺激铵的情况下，可以占DLDH活性。线粒体NADH依赖的过氧化氢生产的生理相关条件下的意义进行了讨论。本文是题为：第17届欧洲生物能学会议（EBEC 2012）的特刊的一部分。(C)2012爱思唯尔有限公司版权所有。
The rates of NADH-supported superoxide/hydrogen peroxide production by membrane-bound bovine heart respiratory complex I. soluble pig heart dihydrolipoamide dehydrogenase (DLDH), and by accompanying operation of these enzymes in rat heart mitochondrial matrix were measured as a function of the pool of pyridine nucleotides and its redox state. Each of the activities showed nontrivial dependence on nucleotide pool concentration. The NAD(+)/NADH ratios required for their half maximal capacities were determined. About half of the total NADH-supported H2O2 production by permeabilized mitochondria in the absence of stimulating ammonium could be accounted for by DLDH activity. The significance of the mitochondrial NADH-dependent hydrogen peroxide production under physiologically relevant conditions is discussed. This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012). (C) 2012 Elsevier B.V. All rights reserved.