Differences in nucleosome organization over episomally located plasmids coincides with aberrant promoter activity in P. falciparum
Differences in nucleosome organization over episomally located plasmids coincides with aberrant promoter activity in P. falciparum
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DOI：
10.1016/s1383-5769(99)00002-1
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发表时间：
1999-03-01
影响因子：
1.9
通讯作者：
Lanzer, Michael
中科院分区：
文献类型：
--
作者：
Horrocks, Paul;Lanzer, Michael
在这里，我们研究了当恶性疟原虫 GBP130 启动子位于使用瞬时转染引入的附加型质粒上时，其是否在红细胞内周期中保持其发育活性。将其活性与内源染色体定位的 GBP130 启动子的活性进行比较表明，附加型定位的 GBP130 启动子失去了其发育限制，变得组成型活性。发育限制的丧失与附加体上不存在定相核小体阵列相一致。这些数据表明表观遗传因素可能在恶性疟原虫的发育调控基因表达中发挥作用。
Here we investigated whether the Plasmodium falciparum GBP130 promoter maintains its developmental activity during the intraerythrocytic cycle when located on an episomal plasmid introduced using transient transfection. Comparing its activity with that of the endogenous chromosomally located GBP130 promoter indicates that the episomally located GBP130 promoter looses its developmental restriction, being rendered constitutively active. Loss of developmental restriction coincides with the absence of phased nucleosomal arrays over the episome. These data suggest that epigenetic factors may play a role in developmentally regulated gene expression in P. falciparum.