Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber
Selective femtosecond laser ablation via two-photon fluorescence imaging through a multimode fiber
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DOI：
10.1364/boe.10.000423
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发表时间：
2019-02-01
影响因子：
3.4
通讯作者：
Psaltis, Demetri
中科院分区：
文献类型：
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作者：
Kakkava, Eirini;Romito, Marilisa;Psaltis, Demetri
我们证明了多模光纤探针提供双光子荧光（TPF）成像反馈的能力，该反馈引导生物样品中的飞秒激光消融（弗拉）进行高度选择性的修改。我们通过梯度折射率（GRIN）多模光纤的高功率飞秒脉冲的传播来实现该系统，我们研究了激光烧蚀所需的高激光峰值强度所带来的限制。我们证明GRIN光纤探头可以提供高达1.5x10（13）W/cm（2）的激光强度。足以烧蚀包括生物样品在内的各种材料。通过直径约400 μ m的双折射探头的波前整形用于衍射极限聚焦和聚焦光斑的数字扫描。基于通过同一多模光纤探头获得的TPF图像进行耳蜗毛细胞的选择性弗拉。(C)根据OSA开放获取出版协议的条款，2019年美国光学学会
We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x10(13) W/cm(2). sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 mu m in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement