Induction of superovulation by immunoneutralization of endogenous inhibin in immature rats
Induction of superovulation by immunoneutralization of endogenous inhibin in immature rats
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DOI：
10.1262/jrd.17020
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发表时间：
2005-10-01
影响因子：
1.8
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Ishigame, H;Medan, MS;Taya, K
研究了被动免疫中和内源性抑制素对幼鼠排卵率的影响。比较了抑制素抗血清(抑制素-AS)和马绒毛膜促性腺激素(CG)方案对受精卵的超数排卵效率。未成熟雌性Wistar大鼠单次注射L抑制素-AS超数排卵，同时注射人绒毛膜促性腺激素(HCG)和不注射hCG。一次注射L抑制素-AS的26-30日龄大鼠72h排卵率为77.8%，而注射正常山羊血清的大鼠未排卵。在28日龄时，当给予额外的hCG处理时，所有抑制素-AS处理的大鼠都排卵，而排卵卵母细胞的数量没有受到影响。抑制素AS-hCG处理组的排卵卵母细胞数显著高于NGS-hCG处理组。此外，抑制素-AS-hCG治疗组的血浆FSH浓度较NGS治疗组显著升高。而200MU L抑制素反义人绒毛膜促性腺激素处理组大鼠的交配率显著低于15IU组，但受精率与对照组相当。200U L抑制素反义人绒毛膜促性腺激素处理组受精卵母细胞数显著高于15IU组。这些结果表明，免疫中和内源性抑制素可能是一种可靠的超数排卵方法，可用于收集大量未成熟大鼠正常受精的卵母细胞。
The effects of passive immunoneutralization of endogenous inhibin on ovulation rate in immature rats were investigated. Efficiency of superovulation on production of fertilized oocytes was compared between the inhibin antiserum (inhibin-AS) and equine chorionic gonadotropin (eCG) protocols. Immature female Wistar strain rats were superovulated with a single injection of 100-200 mu l inhibin-AS, with and without an injection of human chorionic gonadotropin (hCG). A total of 77.8% of the 26-30-day-old rats treated with a single injection of 100-200 mu l inhibin-AS ovulated 72 h after treatment, while rats given normal goat serum (NGS; 200 mu l) did not ovulate. At 28 days of age, all of the inhibin-AS treated rats ovulated when additional hCG treatment was given, whereas the number of ovulated oocytes was not affected. The number of ovulated oocytes in the inhibin-AS-hCG treated groups was significantly higher than that of the NGS-hCG treated group. In addition, plasma concentrations of FSH in the inhibin-AS-hCG treated group significantly increased compared with the NGS treated group. While the percentage of mated rats in the 200 mu l inhibin-AS-hCG treated group was significantly lower than that of the 15 IU eCG-hCG treated group, the fertilization rate was comparable between the two groups. The number of fertilized oocytes in the 200 mu l inhibin-AS-hCG treated group was significantly higher in comparison with the 15 IU eCG-hCG treated group. These results suggest that immunoneutralization of endogenous inhibin could be a reliable method for induction of superovulation to collect a large number of normally fertilized oocytes in immature rats.